ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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产品列表

sp-pme100886 il1ra sp1

ECD Proteins

Human IL1RA Protein, hFc Tag

SKU:  PME100886     Target:  IL1RA     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100885 il17b sp1

ECD Proteins

Human IL17B Protein, hFc Tag

SKU:  PME100885     Target:  IL17B     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100884 il17a sp1

ECD Proteins

Human IL17A Protein, hFc Tag

SKU:  PME100884     Target:  IL17A     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100μg ￥4299.00

sp-pme100555 il21 sp1

ECD Proteins

Human IL21 Protein, mFc Tag

SKU:  PME100555     Target:  IL21     Tag:  C-Mouse Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100882 il15 sp1

ECD Proteins

Human IL15 Protein, hFc Tag

SKU:  PME100882     Target:  IL15     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100881 il12rb1 sp1

ECD Proteins

Human IL12RB1 Protein, His Tag

SKU:  PME100881     Target:  IL12RB1     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100879 il10r sp1

ECD Proteins

Human IL10R Protein, hFc Tag

SKU:  PME100879     Target:  IL10R     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100878 il10 sp1

ECD Proteins

Human IL10 Protein, hFc Tag

SKU:  PME100878     Target:  IL10     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100588 c mpl sp1

ECD Proteins

Human c-MPL Protein, hFc Tag

SKU:  PME100588     Target:  c-MPL     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100872 il13 sp1

ECD Proteins

Human IL13 Protein, hFc Tag

SKU:  PME100872     Target:  IL13     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100871 gnrhr sp1

ECD Proteins

Human GNRHR Protein, hFc Tag

SKU:  PME100871     Target:  GNRHR     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100870 gdnf sp1

ECD Proteins

Human GDNF Protein, hFc Tag

SKU:  PME100870     Target:  GDNF     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100868 folr2 sp1

ECD Proteins

Human FOLR2 Protein, hFc Tag

SKU:  PME100868     Target:  FOLR2     Tag:  C-Human Fc Tag

Price:10μg ￥600.00; 50μg ￥1550.00; 100μg ￥2173.50

sp-pme100867 fcmr sp1

ECD Proteins

Human FCMR Protein, hFc Tag

SKU:  PME100867     Target:  FCMR     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme100863 colec10 sp1

ECD Proteins

Human COLEC10 Protein, hFc Tag

SKU:  PME100863     Target:  COLEC10     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100862 cldn3 sp1

ECD Proteins

Human CLDN3 Protein, hFc Tag

SKU:  PME100862     Target:  CLDN3     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100861 itgav itgb1 sp1

ECD Proteins

Human ITGAV and ITGB1 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME100861     Target:  ITGAV and ITGB1     Tag:  C-6×His Tag and C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100857 prom1 sp1

ECD Proteins

Human PROM1 Protein, hFc Tag

SKU:  PME100857     Target:  PROM1     Tag:  N-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100156 cb2 sp1

ECD Proteins

Human CB2 (1-33) Protein, hFc Tag

SKU:  PME100156     Target:  CB2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100855 vsig4 sp1

ECD Proteins

Human VSIG4 Protein, hFc Tag

SKU:  PME100855     Target:  VSIG4     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme100853 tnfrsf11a sp1

ECD Proteins

Human TNFRSF11A Protein, hFc Tag

SKU:  PME100853     Target:  TNFRSF11A     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100852 ncr1 sp1

ECD Proteins

Human NCR1 Protein, hFc Tag

SKU:  PME100852     Target:  NCR1     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100623 chi3l1 sp1

ECD Proteins

Human CHI3L1 Protein, His Tag

SKU:  PME100623     Target:  CHI3L1     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100604 clu sp1

ECD Proteins

Human CLU Protein, His Tag

SKU:  PME100604     Target:  CLU     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100848 cd99 sp1

ECD Proteins

Human CD99 Protein, hFc Tag

SKU:  PME100848     Target:  CD99     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100321 klrg1 sp1

ECD Proteins

Human KLRG1 Protein, hFc Tag

SKU:  PME100321     Target:  KLRG1     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme100844 kir2dl2 sp1

ECD Proteins

Human KIR2DL2 Protein, hFc Tag

SKU:  PME100844     Target:  KIR2DL2     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100841 il8 sp1 1

ECD Proteins

Human IL8 Protein, hFc Tag

SKU:  PME100841     Target:  IL8     Tag:  N-Human Fc tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100840 il7ra sp1

ECD Proteins

Human IL7RA Protein, hFc Tag

SKU:  PME100840     Target:  IL7RA     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme100838 il1b sp1

ECD Proteins

Human IL1B Protein, hFc Tag

SKU:  PME100838     Target:  IL1B     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100836 lrrc32 sp1

ECD Proteins

Human LRRC32 Protein, hFc Tag

SKU:  PME100836     Target:  LRRC32     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100835 cxcr5 sp1

ECD Proteins

Human CXCR5 Protein, hFc Tag

SKU:  PME100835     Target:  CXCR5     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00