Recombinant human GITR protein with C-terminal mouse Fc and 6×His tag
C-Mouse Fc and 6×His Tag
GITR(Gln26-Pro162) mFc(Pro99-Lys330) 6×His
The protein has a predicted molecular mass of 50-52 kDa after removal of the signal peptide.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Research use only
Human GITR Protein, mFc-His tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00
Figure 1. Human GITR Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR, mFc-His tagged protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.