Synthetic Nanodisc全长多穿膜蛋白

Nanodisc中文名称为纳米圆盘,该平台利用具有疏水和亲水双重特性的物质作为稳定剂,稳定剂朝向内部脂层的疏水面可将膜蛋白整合到Nanodisc中,维持膜蛋白的天然空间构象和活性。同时,朝外的亲水面使得Nanodisc在水溶液中具有很高的溶解度和稳定性。
与市面上大多数MSP Nanodisc不同,缔码生物研发的Synthetic Nanodisc能直接从完整的细胞中制作。在这个过程中,使用的合成高分子具有双重功能。首先,它溶解细胞膜,类似于洗涤剂,同时利用天然细胞磷脂在膜蛋白周围形成纳米盘结构。此外,缔码制备Nanodisc蛋白时使用的是哺乳动物表达系统,这可确保蛋白在结构、功能和翻译后修饰方面与天然对应物非常相似,使其成为生物化学和生物医学领域广泛应用的理想工具。

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案例展示

Human SLC7A11 full length protein-synthetic nanodisc (Cat# FLP100048) 12次跨膜蛋白

Human CLDN18.2 full length protein-synthetic nanodisc (Cat# FLP100014) 4次跨膜蛋白

Human MDR-1 full length protein-synthetic nanodisc (Cat# FLP100029) 12次跨膜蛋白

Human GPRC5D full length protein-synthetic nanodisc (Cat# FLP100011) 7次跨膜蛋白

Human CD63 full length protein-synthetic nanodisc (Cat#: FLP100030)

High Activity

ELISA

High Stability

Lyophilization test

Accelerated Test (Lyophilized Powder)

Synthetic Nanodiscs

Nanodisc Platform

优势:

  • 可以制备高度纯化的膜蛋白
  • 水溶液中具有高的溶解度
  • 高稳定性
  • 跨膜蛋白处于天然膜环境中,保持生物活性
  • 后续实验中不需要添加洗涤剂,可以直接应用于细胞学分析
  • 不含不相关MSP骨架蛋白
  • 哺乳细胞表达系统能保证其翻译后修饰

应用:

  • ELISA
  • SPR affinity analysis
  • Phage display screening
  • Immunization
  • Cell based assays
  • Protein crystal structure analysis

Nanodiscs产品&服务

Nanodisc现货产品

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    Synthetic Nanodiscs Products

    Full Length Transmembrane Proteins

    Human AQP5 full length protein-synthetic nanodisc

    SKU:  FLP100158     Target:  AQP5

    Price: 10 μg ¥6300.00 ; 50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human ADRB1 full length protein-synthetic nanodisc

    SKU:  FLP100157     Target:  ADRB1

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human ABCG1 full length protein-synthetic nanodisc

    SKU:  FLP100156     Target:  ABCG1

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human NPSR1 full length protein-synthetic nanodisc

    SKU:  FLP100369     Target:  NPSR1

    Price: 10 μg ¥6300.00 ; 50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human DRD2 full length protein-synthetic nanodisc

    SKU:  FLP100230     Target:  DRD2

    Price: 10 μg ¥6300.00 ; 50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human 5HT7R full length protein-synthetic nanodisc

    SKU:  FLP100172     Target:  5HT7R

    Price: 10 μg ¥6300.00 ; 50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human TAS1R2 full length protein-synthetic nanodisc

    SKU:  FLP100155     Target:  TAS1R2

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human VN1R1 full length protein-synthetic nanodisc

    SKU:  FLP100495     Target:  VN1R1

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human VIPR2 full length protein-synthetic nanodisc

    SKU:  FLP100494     Target:  VIPR2

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human VIPR1 full length protein-synthetic nanodisc

    SKU:  FLP100493     Target:  VIPR1

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human V2R full length protein-synthetic nanodisc

    SKU:  FLP100492     Target:  V2R

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00

    Full Length Transmembrane Proteins

    Human V1BR full length protein-synthetic nanodisc

    SKU:  FLP100491     Target:  V1BR

    Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00