对于单次穿膜蛋白来说，DIMA可提供功能活性和高稳定性的胞外区重组蛋白（ECD蛋白）。这些蛋白经过广泛测试和验证，以确保其功能完整性，使它们非常适用于药物研究和开发的各种应用场景。
High Purity, High Bioactivity
High Purity, High Bioactivity
Protein Purity
Figure 1.
Human SIRPa, hFc-His Tag (A) and CD47, mFc-His Tag (B) on SDS-PAGE under reducing condition.
Receptor-ligand interaction
Figure 2.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind its native ligand Human SIRPα, hFc-His tagged protein (PME100009) in a linear range of 3.3-26.37 ng/ml.
Biosimilar interaction
Figure 3.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD47, mFc-His tagged protein (PME100008) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) (BME100001) in a linear range of 0.061-1.606 ng/ml.
Freeze-thaw stability testing
Protein Purity
Lane 1: Human SIRPα,
hFc-His tagged protein without freeze-thaw treatment,
Lane 2: Human SIRPα,
hFc-His tagged protein after one freeze-thaw cycle,
Lane 3: Human SIRPα,
hFc-His tagged protein after three freeze-thaw cycles,
Lane 4: Human SIRPα,
hFc-His tagged protein after five freeze-thaw cycles.
Receptor-ligand interaction
A: Human SIRPα,
hFc-His tagged protein without freeze-thaw treatment.
B: Human SIRPα,
hFc-His tagged protein after one freeze-thaw cycle.
C: Human SIRPα,
hFc-His tagged protein after three freeze-thaw cycles.
D: Human SIRPα,
hFc-His tagged protein after five freeze-thaw cycles.
Storage Stability: Shelf Life Testing
ACE2-hFc, 37℃, 1、2、3 days storage stability
Lane 1: Human ACE2, hFc tagged protein after storage at -20℃ ；
Lane 2: Human ACE2, hFc tagged protein after storage at 37℃ after 24h；
Lane 3: Human ACE2, hFc tagged protein after storage at 37℃ after 48h；
Lane 4: Human ACE2, hFc tagged protein after storage at 37℃ after 72h.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) S-RBD, mFc-His tagged protein (PME100497) can bind Human ACE2, hFc Tagged protein (Stored at -20℃ or 37℃ for 24h, 48h and 72h) in a linear range of 0.488-49.83 ng/ml.
CCR8-hFc, 37℃, 1、2、3 days storage stability
Lane 1: Human CCR8, hFc tagged protein after storage at -20℃ ；
Lane 2: Human CCR8, hFc tagged protein after storage at 37℃ after 24h；
Lane 3: Human CCR8, hFc tagged protein after storage at 37℃ after 48h；
Lane 4: Human CCR8, hFc tagged protein after storage at 37℃ after 72h.
ELISA plate pre-coated by 1μg/ml (100μl/well) Human CCR8, hFc Tag ( (Stored at -20℃ or 37℃ for 24h, 48h and 72h) ) can bind Anti-CCR8 Neutralizing antibody (BME100063) in a linear range of 3.2-80 ng/ml.
Storage Stability Testing After Reconstitution
37℃, 1、2、3 days storage stability after reconstitution of lyophilized protein
SDS-PAGE
ELISA
PME100073：Human ACE2 Protein, hFc Tag
SDS-PAGE
ELISA
PME101091：Human CCR8 Protein, hFc Tag
Lot-to-lot consistency
CCR8-hFc lot-to-lot consistency
Different batches of Human CCR8 Protein, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 1μg/ml (100μl/well) different batches Human CCR8, hFc Tag (PME101091) can bind Anti-CCR8 Neutralizing antibody (BME100063) in a linear range of 3.2-80 ng/ml.
