Figure 1： Workflow for BCMA DimAb^® lead molecules project. From 4ml of rabbit whole blood, we identified 5 lead mAbs molecules for human BCMA targets with verified functional data and antibody sequences. We also created a DimAb B cell seed library for the human BCMA target which presumably contains 10 thousands Flow positive BCMA binders providing a good resource for additional screening.

Figure 2: Quality analysis of purified human BCMA protein, Fc-tagged. A. Human BCMA, Fc-tagged on SDS-PAGE under reducing condition. B. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BAFF, hFc tagged protein can bind Human BCMA, Fc- tagged protein in a linear range of 0.03-15.625 ng/ml. C. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA, Fc-tagged protein can bind Anti-BCMA (huC11D5.3) (Its variable region was used to construct scFv portion of CAR-T Idecabtagene vicleucel (bb2121). ) in a linear range of 3.71-22.29 ng/ml.

Figure 3： Phylogenetic analysis of 13 different Anti-BCMA DimAb clones A) heavy chain and B) Light chain. All these clones work for flow application. The boxed regions indicate heavy and light chains of the same clone come from the same lineage group.

Figure 4： Epitope comparison between different anti-BCMA DimAb clones and anti-BCMA huC11D5.3 clone (Bluebird bb2121). ELISA plate was coated with recombinant BCMA-hFc fusion protein, followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar) and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. One clone exhibits the strongest inhibition (Red bar). This data indicated that one clone binds to the same epitope as bb2121.

Figure 6： In-vivo testing of humanized anti-BCMA lead mAbs molecules. The preliminary tumor cell killing efficacy testing data is proprietary. It indicated that our 5 humanized CARs are comparable or better than BMK.