ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

人、鼠、猴、犬源重组蛋白：抗体药物研发的隐藏关键

在抗体药物（therapeutic antibody）研发流程中，…

14
11 月
靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

04
12 月

产品列表

sp-pme101001 rhoc sp1

ECD Proteins

Human RHOC Protein, hFc Tag

SKU:  PME101001     Target:  RhoC     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101000 ptger4 sp1

ECD Proteins

Human PTGER4 Protein, hFc Tag

SKU:  PME101000     Target:  PTGER4     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100999 ptger2 sp1

ECD Proteins

Human PTGER2 Protein, hFc Tag

SKU:  PME100999     Target:  PTGER2     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100981 mmp2 sp1

ECD Proteins

Human MMP2 Protein, His Tag

SKU:  PME100981     Target:  MMP2     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100980 mmp11 sp1

ECD Proteins

Human MMP11 Protein, hFc Tag

SKU:  PME100980     Target:  MMP11     Tag:  C-Human Fc Tag

Price: 10ug ￥1722; 50ug ￥6720, 100ug ￥10080

sp-PME100977 MERTK hFc PAGE 72

ECD Proteins

Human MERTK Protein, hFc Tag

SKU:  PME100977     Target:  MERTK     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100580 app sp1

ECD Proteins

Human APP Protein, His Tag

SKU:  PME100580     Target:  APP     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100970 kras sp1

ECD Proteins

Human KRAS Protein, hFc Tag

SKU:  PME100970     Target:  KRAS     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100962 ifngr2 sp1

ECD Proteins

Human IFNGR2 Protein, hFc Tag

SKU:  PME100962     Target:  IFNGR2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100959 ifnar2 sp1

ECD Proteins

Human IFNAR2 Protein, hFc Tag

SKU:  PME100959     Target:  IFNAR2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100958 ifnar1 sp1

ECD Proteins

Human IFNAR1 Protein, His Tag

SKU:  PME100958     Target:  IFNAR1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100952 lgals1 sp1

ECD Proteins

Human LGALS1 Protein, hFc Tag

SKU:  PME100952     Target:  LGALS1     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100947 gast sp1

ECD Proteins

Human GAST Protein, hFc Tag

SKU:  PME100947     Target:  GAST     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100945 fn1 sp1

ECD Proteins

Human FN1 Protein, His Tag

SKU:  PME100945     Target:  FN1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100941 egf sp1

ECD Proteins

Human EGF Protein, hFc Tag

SKU:  PME100941     Target:  EGF     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100250 folr2 sp1

ECD Proteins

Human FOLR2 Protein, mFc Tag

SKU:  PME100250     Target:  FOLR2     Tag:  C-mouse Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100μg ￥4299.00

sp-pme100937 cxcr2 sp1

ECD Proteins

Human CXCR2 Protein, hFc Tag

SKU:  PME100937     Target:  CXCR2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100936 cxcl9 sp1

ECD Proteins

Human CXCL9 Protein, hFc Tag

SKU:  PME100936     Target:  CXCL9     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100934 cxcl1 sp1

ECD Proteins

Human CXCL1 Protein, hFc Tag

SKU:  PME100934     Target:  CXCL1     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100930 gm csfr sp1

ECD Proteins

Human GM-CSFR Protein, hFc Tag

SKU:  PME100930     Target:  GM-CSFR     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100923 ccr5 sp1

ECD Proteins

Human CCR5 Protein, hFc Tag

SKU:  PME100923     Target:  CCR5     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100922 ccr2 sp1

ECD Proteins

Human CCR2 Protein, hFc Tag

SKU:  PME100922     Target:  CCR2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100921 ccr1 sp1

ECD Proteins

Human CCR1 Protein, hFc Tag

SKU:  PME100921     Target:  CCR1     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100560 gprc5d sp1

ECD Proteins

Mouse GPRC5D Protein, hFc Tag

SKU:  PME100560     Target:  GPRC5D     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100919 ccl2 sp1

ECD Proteins

Human CCL2 Protein, hFc Tag

SKU:  PME100919     Target:  CCL2     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100917 cb2 sp1

ECD Proteins

Human CB2 Protein, hFc Tag

SKU:  PME100917     Target:  CB2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100916 calr sp1

ECD Proteins

Human CALR Protein, His Tag

SKU:  PME100916     Target:  CALR     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100915 brd4 sp1

ECD Proteins

Human BRD4 Protein, hFc Tag

SKU:  PME100915     Target:  BRD4     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100619 gas6 sp1

ECD Proteins

Human GAS6 Protein, His Tag

SKU:  PME100619     Target:  GAS6     Tag:  C-6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme100128 adfam9 sp1

ECD Proteins

Human ADAM9 Protein, hFc Tag

SKU:  PME100128     Target:  ADAM9     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100895 magea4 sp1

ECD Proteins

Human MAGEA4 Protein, His Tag

SKU:  PME100895     Target:  MAGEA4     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100556 il21 sp1

ECD Proteins

Human IL21 Protein, His Tag

SKU:  PME100556     Target:  IL21     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00