ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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产品列表

sp-pme101085 cd43 sp1

ECD Proteins

Human CD43 Protein, hFc Tag

SKU:  PME101085     Target:  CD43     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme101083 il20 sp1

ECD Proteins

Human IL20 Protein, hFc Tag

SKU:  PME101083     Target:  IL20     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101080 ccr3 sp1

ECD Proteins

Human CCR3 Protein, hFc Tag

SKU:  PME101080     Target:  CCR3     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101079 il18ra sp1

ECD Proteins

Human IL18RA Protein, hFc Tag

SKU:  PME101079     Target:  IL18RA     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101078 ct83 sp1

ECD Proteins

Human CT83 Protein, hFc Tag

SKU:  PME101078     Target:  CT83     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101076 gpc1 sp1

ECD Proteins

Human GPC1 Protein, His Tag

SKU:  PME101076     Target:  GPC1     Tag:  C-7×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101075 cxcl16 sp1

ECD Proteins

Human CXCL16 Protein, hFc Tag

SKU:  PME101075     Target:  CXCL16     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101070 tm4sf1 sp1

ECD Proteins

Human TM4SF1 (31-49) Protein, hFc Tag

SKU:  PME101070     Target:  TM4SF1     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101068 il2rg sp1

ECD Proteins

Human IL2RG Protein, hFc Tag

SKU:  PME101068     Target:  IL2RG     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101067 ceacam8 sp1

ECD Proteins

Human CEACAM8 Protein, hFc Tag

SKU:  PME101067     Target:  CEACAM8     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101060 cxcl13 sp1

ECD Proteins

Human CXCL13 Protein, hFc Tag

SKU:  PME101060     Target:  CXCL13     Tag:  N-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101058 ifngr1 sp1

ECD Proteins

Human IFNGR1 Protein, hFc Tag

SKU:  PME101058     Target:  IFNGR1     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101055 il18 sp1

ECD Proteins

Human IL18 Protein, hFc Tag

SKU:  PME101055     Target:  IL18     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100208 clec2d sp1

ECD Proteins

Human CLEC2D Protein, N-His Tag

SKU:  PME100208     Target:  CLEC2D     Tag:  N-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101051 fgl1 sp1

ECD Proteins

Human FGL1 Protein, hFc Tag

SKU:  PME101051     Target:  FGL1     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101050 cd24 sp1

ECD Proteins

Human CD24 Protein, hFc Tag

SKU:  PME101050     Target:  CD24     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101045 faslg sp1

ECD Proteins

Human FASLG Protein, hFc Tag

SKU:  PME101045     Target:  FASLG     Tag:  N-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100603 clec14a sp1

ECD Proteins

Human CLEC14A Protein, His Tag

SKU:  PME100603     Target:  CLEC14A     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100602 cdcp1 sp1

ECD Proteins

Human CDCP1 Protein, His Tag

SKU:  PME100602     Target:  CDCP1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme101041 b7 h5 sp1

ECD Proteins

Human B7-H5 Protein, hFc Tag

SKU:  PME101041     Target:  B7-H5     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101039 cd6 sp1

ECD Proteins

Human CD6 Protein, His Tag

SKU:  PME101039     Target:  CD6     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100557 il2 sp1

ECD Proteins

Human IL2 Protein, His Tag

SKU:  PME100557     Target:  IL2     Tag:  C-6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme101032 flt1 sp1

ECD Proteins

Human FLT1 Protein, hFc Tag

SKU:  PME101032     Target:  FLT1     Tag:  C-Human Fc Tag

Price: 10μg ￥854.00 ; 50μg ￥3133.00 ; 100 μg ￥4699.00

sp-pme101025 tnfrsf1a sp1

ECD Proteins

Human TNFRSF1A Protein, hFc Tag

SKU:  PME101025     Target:  TNFRSF1A     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100593 ptn sp1

ECD Proteins

Human PTN Protein, hFc Tag

SKU:  PME100593     Target:  PTN     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101020 tlr3 sp1

ECD Proteins

Human TLR3 Protein, hFc Tag

SKU:  PME101020     Target:  TLR3     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100592 fgfr4 sp1

ECD Proteins

Human FGFR4 Protein, His Tag

SKU:  PME100592     Target:  FGFR4     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101017 tgfbr2 sp1

ECD Proteins

Human TGFBR2 Protein, hFc Tag

SKU:  PME101017     Target:  TGFBR2     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101015 tgfb2 sp1

ECD Proteins

Human TGFB2(303-414) Protein, hFc Tag

SKU:  PME101015     Target:  TGFB2     Tag:  N-Human Fc tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101008 spam1 sp1

ECD Proteins

Human SPAM1 Protein, hFc Tag

SKU:  PME101008     Target:  SPAM1     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101006 siglec9 sp1

ECD Proteins

Human SIGLEC9 Protein, His Tag

SKU:  PME101006     Target:  SIGLEC9     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme101003 robo1 sp1

ECD Proteins

Human ROBO1 Protein, hFc Tag

SKU:  PME101003     Target:  ROBO1     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00