ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

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产品列表

sp-pme101969 klk2 sp1

ECD Proteins

Human KLK2 Protein, His Tag

SKU:  PME101969     Target:  KLK2     Tag:  C-10×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101966 klk2 sp1

ECD Proteins

Human KLK2 Protein, hFc Tag

SKU:  PME101966     Target:  KLK2     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme c100106 il11 sp1

ECD Proteins

Cynomolgus IL11 Protein, hFc Tag

SKU:  PME-C100106     Target:  IL11     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100105 il11ra sp1

ECD Proteins

Cynomolgus IL11RA Protein, His Tag

SKU:  PME-C100105     Target:  IL11RA     Tag:  C-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100104 st2 sp1

ECD Proteins

Cynomolgus ST2 Protein, His Tag

SKU:  PME-C100104     Target:  ST2     Tag:  C-10×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme c100103 osmr sp1

ECD Proteins

Cynomolgus OSMR Protein, His Tag

SKU:  PME-C100103     Target:  OSMR     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme c100102 ceacam8 sp1

ECD Proteins

Cynomolgus CEACAM8 Protein, His Tag

SKU:  PME-C100102     Target:  CEACAM8     Tag:  C-10×His tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme c100101 pd 1 sp1

ECD Proteins

Cynomolgus PD-1 Protein, hFc Tag

SKU:  PME-C100101     Target:  PD-1     Tag:  C-Human Fc tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme c100100 ox40 sp1

ECD Proteins

Cynomolgus OX40 Protein, hFc Tag

SKU:  PME-C100100     Target:  OX40     Tag:  C-Human Fc tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme c100099 4 1bb ligand sp1

ECD Proteins

Cynomolgus 4-1BB Ligand Protein, hFc Tag

SKU:  PME-C100099     Target:  4-1BB Ligand     Tag:  N-Human Fc tag

Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00

sp-pme c100098 sirpα sp1

ECD Proteins

Cynomolgus SIRPα Protein, hFc Tag

SKU:  PME-C100098     Target:  SIRPα     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme c100097 sirpα sp1

ECD Proteins

Cynomolgus SIRPα Protein, His Tag

SKU:  PME-C100097     Target:  SIRPα     Tag:  C-10×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme m100136 st2 sp1

ECD Proteins

Mouse ST2 Protein, His Tag

SKU:  PME-M100136     Target:  ST2     Tag:  C-10×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme m100135 fap sp1

ECD Proteins

Mouse FAP Protein, His Tag

SKU:  PME-M100135     Target:  FAP     Tag:  N-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme m100134 cxcr3 sp1

ECD Proteins

Mouse CXCR3 Protein, hFc Tag

SKU:  PME-M100134     Target:  CXCR3     Tag:  C-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme m100133 cd94 sp1

ECD Proteins

Mouse CD94 Protein, hFc Tag

SKU:  PME-M100133     Target:  CD94     Tag:  N-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme m100132 crtam sp1

ECD Proteins

Mouse CRTAM Protein, His Tag

SKU:  PME-M100132     Target:  CRTAM     Tag:  C-10×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme m100129 cxcr2 sp1

ECD Proteins

Mouse CXCR2(1-47) Protein, hFc Tag

SKU:  PME-M100129     Target:  CXCR2     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101962 steap1 sp1

ECD Proteins

Human STEAP1(312-339) Protein, hFc Tag

SKU:  PME101962     Target:  STEAP1     Tag:  N-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101961 steap1 sp1

ECD Proteins

Human STEAP1(1-70) Protein, hFc Tag

SKU:  PME101961     Target:  STEAP1     Tag:  N-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101960 cd1d sp1

ECD Proteins

Human CD1D Protein, His Tag

SKU:  PME101960     Target:  CD1D     Tag:  C-6×His tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101959 crtam sp1

ECD Proteins

Human CRTAM(211-287) Protein, hFc Tag

SKU:  PME101959     Target:  CRTAM     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101958 crtam sp1

ECD Proteins

Human CRTAM(18-117) Protein, hFc Tag

SKU:  PME101958     Target:  CRTAM     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101957 crtam sp1

ECD Proteins

Human CRTAM(115-287) Protein, hFc Tag

SKU:  PME101957     Target:  CRTAM     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme101954 ly6g6d sp1

ECD Proteins

Human LY6G6D Protein, hFc Tag

SKU:  PME101954     Target:  LY6G6D     Tag:  N-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101953 snca sp1

ECD Proteins

Human SNCA Protein, hFc Tag

SKU:  PME101953     Target:  SNCA     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101952 gpr88 sp1

ECD Proteins

Human GPR88 Protein, hFc Tag

SKU:  PME101952     Target:  GPR88     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101951 tfrc sp1

ECD Proteins

Human TFRC(121-760) Protein, His Tag

SKU:  PME101951     Target:  TFRC     Tag:  N-10×His tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101950 sirpα sp1

ECD Proteins

Human SIRPα V8 Protein, His Tag

SKU:  PME101950     Target:  SIRPα     Tag:  C-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101949 sirpα sp1

ECD Proteins

Human SIRPα V8 Protein, mFc Tag

SKU:  PME101949     Target:  SIRPα     Tag:  C-Mouse Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101948 sirpα sp1

ECD Proteins

Human SIRPα V2 Protein, mFc Tag

SKU:  PME101948     Target:  SIRPα     Tag:  C-Mouse Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101947 sirpα sp1

ECD Proteins

Human SIRPα V2 Protein, His Tag

SKU:  PME101947     Target:  SIRPα     Tag:  C-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00