ECD 蛋白（Extracellular Domain Proteins）

ECD 蛋白（Extracellular Domain Proteins，胞外结构域蛋白）是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。靶点包括GPCR、免疫检查点、肿瘤相关抗原及代谢受体等多个热门可成药靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用，为膜蛋白药物研发提供高质量研究材料。

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

通过去除跨膜结构并优化表达体系，可获得可溶、正确折叠的ECD重组蛋白，既能保留关键抗原表位，又便于ELISA、SPR、流式检测、抗体筛选及结构分析等多种实验应用。随着结构生物学与蛋白工程技术的发展，ECD蛋白已广泛应用于抗体药物发现、受体-配体互作研究以及疫苗设计等领域。

ECD蛋白的应用

  • 抗体初筛（Primary Screening）
  • 抗体亲和力比较与排序
  • 表位分组与机制研究
  • ELISA/SPR/BLI方法建立
  • 全长膜蛋白或细胞实验前的验证步骤

缔码ECD蛋白优势

  • 哺乳动物细胞表达，构象更稳定，筛选更可靠
  • 多物种、多片段现货，快速匹配不同研究需求
  • 1600+现货ECD蛋白，覆盖900+药物靶点
  • 支持流式抗体开发，500+项目实际应用验证
  • 适配多种应用场景，加速抗体开发流程

ECD蛋白热门分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

ECD蛋白数据展示

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

The List of ECD Proteins

elisa-pme d100012 il31 elisa1

ECD Proteins

Canine IL31 Protein, hFc Tag

SKU:  PME-D100012     Target:  IL31     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

elisa-pme d100011 il31 elisa1

ECD Proteins

Canine IL31 Protein, mFc Tag

SKU:  PME-D100011     Target:  IL31     Tag:  C-Mouse Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme c100096 ccr1 sp1

ECD Proteins

Cynomolgus CCR1 Protein, hFc Tag

SKU:  PME-C100096     Target:  CCR1     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme c100094 klrg1 sp1

ECD Proteins

Cynomolgus KLRG1 Protein, hFc Tag

SKU:  PME-C100094     Target:  KLRG1     Tag:  N-Human Fc tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme m100131 cd8a sp1

ECD Proteins

Mouse CD8A Protein, hFc Tag

SKU:  PME-M100131     Target:  CD8A     Tag:  C-Human Fc tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme m100130 ccr1 sp1

ECD Proteins

Mouse CCR1 Protein, hFc Tag

SKU:  PME-M100130     Target:  CCR1     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme m100128 cxcr1 sp1

ECD Proteins

Mouse CXCR1(1-44) Protein, hFc Tag

SKU:  PME-M100128     Target:  CXCR1     Tag:  C-Human Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100127 klrg1 sp1

ECD Proteins

Mouse KLRG1 Protein, hFc Tag

SKU:  PME-M100127     Target:  KLRG1     Tag:  N-Human Fc tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme101932 gpr17 sp1

ECD Proteins

Human GPR17 Protein, hFc Tag

SKU:  PME101932     Target:  GPR17     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101931 gpr15 sp1

ECD Proteins

Human GPR15 Protein, hFc Tag

SKU:  PME101931     Target:  GPR15     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101929 or10j5 sp1

ECD Proteins

Human OR10J5 Protein, hFc Tag

SKU:  PME101929     Target:  OR10J5     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101926 il15 il15ra sp1

ECD Proteins

Human IL15RA and IL15 Protein, mFc Tag

SKU:  PME101926     Target:  IL15 and IL15RA     Tag:  C-Mouse Fc tag and tag Free

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101924 fam171a2 sp1

ECD Proteins

Human FAM171A2 Protein, His Tag

SKU:  PME101924     Target:  FAM171A2     Tag:  C-10×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101923 htr4 sp1

ECD Proteins

Human HTR4 Protein, hFc Tag

SKU:  PME101923     Target:  HTR4     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101922 htr1b sp1

ECD Proteins

Human HTR1B Protein, hFc Tag

SKU:  PME101922     Target:  HTR1B     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101921 hrh1 sp1

ECD Proteins

Human HRH1 Protein, hFc Tag

SKU:  PME101921     Target:  HRH1     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101920 hcrtr2 sp1

ECD Proteins

Human HCRTR2 Protein, hFc Tag

SKU:  PME101920     Target:  HCRTR2     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101919 hcar2 sp1

ECD Proteins

Human HCAR2 Protein, hFc Tag

SKU:  PME101919     Target:  HCAR2     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101918 gpr183 sp1

ECD Proteins

Human GPR183 Protein, hFc Tag

SKU:  PME101918     Target:  GPR183     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101917 gpr161 sp1

ECD Proteins

Human GPR161 Protein, hFc Tag

SKU:  PME101917     Target:  GPR161     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101916 gpr143 sp1

ECD Proteins

Human GPR143 Protein, hFc Tag

SKU:  PME101916     Target:  GPR143     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101915 gpr101 sp1

ECD Proteins

Human GPR101 Protein, hFc Tag

SKU:  PME101915     Target:  GPR101     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101914 gpr18 sp1

ECD Proteins

Human GPR18 Protein, hFc Tag

SKU:  PME101914     Target:  GPR18     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101913 il6st sp1

ECD Proteins

Human IL6ST Protein, His Tag

SKU:  PME101913     Target:  IL6ST     Tag:  C-10×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101912 ffar3 sp1

ECD Proteins

Human FFAR3 Protein, hFc Tag

SKU:  PME101912     Target:  FFAR3     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101911 f2rl2 sp1

ECD Proteins

Human F2RL2 Protein, hFc Tag

SKU:  PME101911     Target:  F2RL2     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101910 ednra sp1

ECD Proteins

Human EDNRA Protein, hFc Tag

SKU:  PME101910     Target:  EDNRA     Tag:  C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101909 drd4 sp1

ECD Proteins

Human DRD4 Protein, hFc Tag

SKU:  PME101909     Target:  DRD4     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101908 drd3 sp1

ECD Proteins

Human DRD3 Protein, hFc Tag

SKU:  PME101908     Target:  DRD3     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101907 drd1 sp1

ECD Proteins

Human DRD1 Protein, hFc Tag

SKU:  PME101907     Target:  DRD1     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101906 crhr2 sp1

ECD Proteins

Human CRHR2 Protein, hFc Tag

SKU:  PME101906     Target:  CRHR2     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101905 chrm3 sp1

ECD Proteins

Human CHRM3 Protein, hFc Tag

SKU:  PME101905     Target:  CHRM3     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00