ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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产品列表

sp-pme100834 cxcr4 sp1

ECD Proteins

Human CXCR4 Protein, hFc Tag

SKU:  PME100834     Target:  CXCR4     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100833 cxcr3 sp1

ECD Proteins

Human CXCR3 Protein, hFc Tag

SKU:  PME100833     Target:  CXCR3     Tag:  C-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme100832 clec2d sp1

ECD Proteins

Human CLEC2D Protein, hFc Tag

SKU:  PME100832     Target:  CLEC2D     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme100831 ceacam1 sp1

ECD Proteins

Human CEACAM1 Protein,His Tag

SKU:  PME100831     Target:  CEACAM1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100830 c5ar1 sp1

ECD Proteins

Human C5AR1 Protein, hFc Tag

SKU:  PME100830     Target:  C5AR1     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100829 cd73 sp1

ECD Proteins

Human CD73 Protein, hFc Tag

SKU:  PME100829     Target:  CD73     Tag:  C-Human Fc Tag

Price: 10μg ￥854.00 ; 50μg ￥3133.00 ; 100 μg ￥4699.00

sp-pme100828 cd3e sp1

ECD Proteins

Human CD3E Protein, hFc Tag

SKU:  PME100828     Target:  CD3E     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100827 cd200r1 sp1

ECD Proteins

Human CD200R1 Protein, His Tag

SKU:  PME100827     Target:  CD200R1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100826 ccr9 sp1

ECD Proteins

Human CCR9 Protein, hFc Tag

SKU:  PME100826     Target:  CCR9     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100825 ccr7 sp1

ECD Proteins

Human CCR7 Protein, hFc Tag

SKU:  PME100825     Target:  CCR7     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100824 ccr4 sp1

ECD Proteins

Human CCR4 (1-39) Protein, hFc Tag

SKU:  PME100824     Target:  CCR4     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100822 ceacam6 sp1

ECD Proteins

Human CEACAM6 Protein, His Tag

SKU:  PME100822     Target:  CEACAM6     Tag:  C-6×His Tag

Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00

sp-pme100821 mst1r sp1

ECD Proteins

Human MST1R Protein, hFc Tag

SKU:  PME100821     Target:  MST1R     Tag:  C-Human Fc Tag

Price: 10μg ￥854.00 ; 50μg ￥3133.00 ; 100 μg ￥4699.00

sp-pme100820 cxcr1 sp1

ECD Proteins

Human CXCR1 Protein, hFc Tag

SKU:  PME100820     Target:  CXCR1     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100818 cd39 sp1

ECD Proteins

Human CD39 Protein, hFc Tag

SKU:  PME100818     Target:  CD39     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100813 cd21 sp1

ECD Proteins

Human CD21 Protein, hFc Tag

SKU:  PME100813     Target:  CD21     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100812 cd147 sp1

ECD Proteins

Human CD147 Protein, hFc Tag

SKU:  PME100812     Target:  CD147     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100810 anpep sp1

ECD Proteins

Human ANPEP Protein, His Tag

SKU:  PME100810     Target:  ANPEP     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100809 il4ra sp1

ECD Proteins

Human IL4RA Protein, hFc Tag

SKU:  PME100809     Target:  IL4RA     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

products-pme

ECD Proteins

Human ITGAM Protein, hFc Tag

SKU:  PME100808     Target:  ITGAM     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme100803 gpa33 sp1

ECD Proteins

Human GPA33 Protein, hFc Tag

SKU:  PME100803     Target:  GPA33     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100802 cdh3 sp1

ECD Proteins

Human CDH3 Protein, His Tag

SKU:  PME100802     Target:  CDH3     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100801 cdh17 sp1

ECD Proteins

Human CDH17 Protein, His Tag

SKU:  PME100801     Target:  CDH17     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100192 cd40 ligand sp1

ECD Proteins

Human CD40 Ligand Protein, hFc Tag

SKU:  PME100192     Target:  CD40 Ligand     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100103 tgfbr1 sp1

ECD Proteins

Human TGFBR1 Protein, N-hFc Tag

SKU:  PME100103     Target:  TGFBR1     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100102 cd46 sp1

ECD Proteins

Human CD46 Protein, His Tag

SKU:  PME100102     Target:  CD46     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100099 egfr sp1

ECD Proteins

Human EGFR Protein, His Tag

SKU:  PME100099     Target:  EGFR     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100098 il2ra sp1

ECD Proteins

Human IL2RA Protein, His Tag

SKU:  PME100098     Target:  IL2RA     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100097 ror2 sp1

ECD Proteins

Human ROR2 Protein, His Tag

SKU:  PME100097     Target:  ROR2     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100096 clec12a sp1

ECD Proteins

Human CLEC12A Protein, His Tag

SKU:  PME100096     Target:  CLEC12A     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100095 her2 sp1

ECD Proteins

Human Her2 Protein, His Tag

SKU:  PME100095     Target:  Her2     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100094 taci sp1

ECD Proteins

Human TACI Protein, hFc Tag

SKU:  PME100094     Target:  TACI     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00