Recombinant Human EPHA2 Protein with C-terminal 6×His tag
EPHA2 (Ala24-Asn534) 6×His tag
The protein has a predicted molecular mass of 57.0 kDa after removal of the signal peptide. The apparent molecular mass of EPHA2-His is approximately 55-70 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.[provided by RefSeq, May 2010]
Research use only
Human EPHA2 Protein, His Tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00
Figure 1. Human EPHA2 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human EPHA2 protein, His Tag (PME100238) can bind Anti-EPHA2 Rabbit mAb in a linear range of 0.128-16 ng/mL.