Recombinant Human B7-1 Protein with C-terminal Mouse Fc and 6×His tag
C-Mouse Fc and 6×His Tag
B7-1(Val35-Asn242) mFc(Pro99-Lys330) 6×His tag
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.
Research use only
Human B7-1 Protein, mFc-His Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
Figure 1. Human B7-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human B7-1, mFc-His tagged protein (PME100047) can bind anti-B7-1 monoclonal antibody,Rabbit mAb (clone: DM111) in a linear range of 0.13-80 ng/ml.
Figure 3. Flow cytometry analysis with 15 μg/mL Human B7-1 Protein, mFc-His tag (PME100047) on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).