Recombinant human VEGFR2 Protein with C-terminal 6×His tag
VEGFR2(Ala20-Glu764) 6×His tag
The protein has a predicted molecular mass of 84.1kDa after removal of the signal peptide.The apparent molecular mass of VEGFR2-His is approximately 130-250 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq, May 2009]
Research use only
Human VEGFR2 Protein, His Tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00
Figure 1. Human VEGFR2 Protein, His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 5 μg/mL (100 μL/well) Human VEGFR2 protein, His Tag (PME100104) can bind Anti-VEGFR2 Neutralizing antibody (BME100060) in a linear range of 6.4-800 ng/mL.