ECD 蛋白（Extracellular Domain Proteins，胞外结构域蛋白）是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。靶点包括GPCR、免疫检查点、肿瘤相关抗原及代谢受体等多个热门可成药靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用，为膜蛋白药物研发提供高质量研究材料。
ECD 蛋白（Extracellular Domain Proteins）
什么是ECD蛋白？
ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。
通过去除跨膜结构并优化表达体系，可获得可溶、正确折叠的ECD重组蛋白，既能保留关键抗原表位，又便于ELISA、SPR、流式检测、抗体筛选及结构分析等多种实验应用。随着结构生物学与蛋白工程技术的发展，ECD蛋白已广泛应用于抗体药物发现、受体-配体互作研究以及疫苗设计等领域。
ECD蛋白的应用
- 抗体初筛（Primary Screening）
- 抗体亲和力比较与排序
- 表位分组与机制研究
- ELISA/SPR/BLI方法建立
- 全长膜蛋白或细胞实验前的验证步骤
缔码ECD蛋白优势
- 哺乳动物细胞表达，构象更稳定，筛选更可靠
- 多物种、多片段现货，快速匹配不同研究需求
- 1600+现货ECD蛋白，覆盖900+药物靶点
- 支持流式抗体开发，500+项目实际应用验证
- 适配多种应用场景，加速抗体开发流程
ECD蛋白热门分类
ECD蛋白数据展示
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.
Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.
Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
The List of ECD Proteins
ECD Proteins
SKU: PME100609 Target: CLEC1A Tag: N-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
100μg 50% 0FF
ECD Proteins
SKU: PME100530 Target: CD38 Tag: C-Human Fc tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00
ECD Proteins
SKU: PME100533 Target: AXL Tag: C-Mouse Fc Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME101102 Target: CD32a Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101099 Target: SCGB2A2 Tag: C-Human Fc tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101098 Target: ADAM17 Tag: C-Human Fc tag
Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00
ECD Proteins
SKU: PME100967 Target: ITGB5 Tag: C-Human Fc tag
Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME100935 Target: CXCL12 Tag: N-Human Fc Tag
Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00
ECD Proteins
SKU: PME100859 Target: CD1A Tag: C-Human Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME100806 Target: SSTR2 Tag: C-Human Fc Tag
Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00
100μg 50% 0FF
ECD Proteins
SKU: PME100481 Target: CD30 Tag: C-6×His Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
ECD Proteins
SKU: PME100018 Target: GITR Tag: C-Human Fc and 6×His Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
ECD Proteins
SKU: PME100009 Target: SIRPa Tag: C-Human Fc and 6×His Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME44614 Target: Mrc2 Tag: C-MYC/DDK
Price: 20 μg ￥2900.00
ECD Proteins
SKU: PME101210 Target: AKT1 Tag: C-8×His Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME30080 Target: CCL16 Tag:
Price: 10 μg ￥936.00 ; 50 μg ￥2800.00
ECD Proteins
SKU: PME30045 Target: IL-29 Tag: C-10×His Tag
Price: 10 μg ￥600.00 ; 50 μg ￥1800.00
ECD Proteins
SKU: PME101097 Target: CD11 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101096 Target: LALBA Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101095 Target: MR1 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101084 Target: FMNL1 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101082 Target: LILRA5 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101074 Target: GPC2 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101073 Target: CD1D Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101072 Target: TIE2 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101071 Target: CD305 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101066 Target: ULBP2 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101061 Target: PPT Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101059 Target: DLK1 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101057 Target: TGFA Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101043 Target: RET Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101028 Target: TYRO3 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
