ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-pme100014 cd28 sp1

ECD Proteins

Human CD28 Protein, mFc-His Tag

SKU:  PME100014     Target:  CD28     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme100013 cd27 sp1

ECD Proteins

Human CD27 Protein, mFc-His Tag

SKU:  PME100013     Target:  CD27     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme100012 b7 h3 sp1

ECD Proteins

Human B7-H3 Protein, mFc-His Tag

SKU:  PME100012     Target:  B7-H3     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100010 2b4 sp1

ECD Proteins

Human 2B4 Protein, mFc-His Tag

SKU:  PME100010     Target:  2B4     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100036 s rbd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag

SKU:  PME100036     Target:  S protein RBD     Tag:  C-6×His Tag

Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00

sp-pme100072 ace2 sp1

ECD Proteins

Human ACE2 Protein, mFc Tag

SKU:  PME100072     Target:  ACE2     Tag:  C-Mouse Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100667 gfap sp1

ECD Proteins

Human GFAP (68-377) Protein, His Tag

SKU:  PME100667     Target:  GFAP     Tag:  N-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100657 nucleocapsid sp1

ECD Proteins

SARS-CoV-2 (Delta) Nucleocapsid, C-His Tag

SKU:  PME100657     Target:  Nucleocapsid     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100670 cd166 sp1

ECD Proteins

Human CD166 Protein, His Tag

SKU:  PME100670     Target:  CD166     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100671 rspo3 sp1

ECD Proteins

Human RSPO3 (22-146) Protein, hFc Tag

SKU:  PME100671     Target:  RSPO3     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100655 folr2 sp1

ECD Proteins

Human FOLR2 Protein, His Tag

SKU:  PME100655     Target:  FOLR2     Tag:  C-6×His Tag

Price:10μg ￥600.00; 50μg ￥1550.00; 100μg ￥2173.50

sp-pme100649 kras sp1

ECD Proteins

Human KRAS Protein, His Tag

SKU:  PME100649     Target:  KRAS     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100648 nefl sp1

ECD Proteins

Human NEFL (89-400) Protein, His Tag

SKU:  PME100648     Target:  NEFL     Tag:  N-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100647 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Delta) S protein RBD, His Tag

SKU:  PME100647     Target:  S protein RBD     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100008 cd47 sp1

ECD Proteins

Human CD47 Protein, mFc-His Tag

SKU:  PME100008     Target:  CD47     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100007 flt3 sp1

ECD Proteins

Human FLT3 Protein, hFc-His Tag

SKU:  PME100007     Target:  FLT3     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100006 cd138 sp1

ECD Proteins

Human CD138 Protein, hFc-His Tag

SKU:  PME100006     Target:  CD138     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100005 cd22 sp1

ECD Proteins

Human CD22 Protein, hFc-His Tag

SKU:  PME100005     Target:  CD22     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100004 cd38 sp1

ECD Proteins

Human CD38 Protein, hFc-His Tag

SKU:  PME100004     Target:  CD38     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100003 cd123 sp1

ECD Proteins

Human CD123 Protein, hFc-His Tag

SKU:  PME100003     Target:  CD123     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100002 cs1 sp1

ECD Proteins

Human CS1 Protein, hFc-His Tag

SKU:  PME100002     Target:  CS1     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100001 bcma sp1

ECD Proteins

Human BCMA Protein, hFc-His Tag

SKU:  PME100001     Target:  BCMA     Tag:  C-Human Fc and 6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-PME100849 CD9 Fig.1 SDS PAGE 1

ECD Proteins

Human CD9 Protein, His Tag

SKU:  PME100849     Target:  CD9     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-PME100609 CLEC1A hFc PAGE 72

ECD Proteins

Human CLEC1A Protein, hFc Tag

SKU:  PME100609     Target:  CLEC1A     Tag:  N-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-PME100530 CD38 Fig.1 SDS PAGE 1

ECD Proteins

Human CD38 Protein, hFc Tag

SKU:  PME100530     Target:  CD38     Tag:  C-Human Fc tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-PME100533 AXL mFc PAGE 72

ECD Proteins

Human AXL Protein, mFc Tag

SKU:  PME100533     Target:  AXL     Tag:  C-Mouse Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-PME101102 CD32a R167 hFc PAGE 300 1

ECD Proteins

Human CD32a (R167) Protein, hFc Tag

SKU:  PME101102     Target:  CD32a     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme101099 scgb2a2 sp1

ECD Proteins

Human SCGB2A2 Protein, hFc Tag

SKU:  PME101099     Target:  SCGB2A2     Tag:  C-Human Fc tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-PME101098 ADAM17 Fig.1 SDS PAGE 1

ECD Proteins

Human ADAM17(581-642) Protein, hFc Tag

SKU:  PME101098     Target:  ADAM17     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME100967 ITGB5 Fig.1 SDS PAGE 1

ECD Proteins

Human ITGB5 Protein, hFc Tag

SKU:  PME100967     Target:  ITGB5     Tag:  C-Human Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME100935 CXCL12 Fig.1 SDS PAGE 1

ECD Proteins

Human CXCL12 Protein, hFc Tag

SKU:  PME100935     Target:  CXCL12     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100859 cd1a sp1

ECD Proteins

Human CD1A Protein, hFc Tag

SKU:  PME100859     Target:  CD1A     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00