| Target | |
|---|---|
| Synonyms | IL-1;IL1-BETA;IL1beta;IL1F2 |
| Description | Recombinant Human IL1B Protein with C-terminal human Fc tag |
| Delivery | In Stock |
| Uniprot ID | P01584 |
| Expression Host | HEK293 |
| Tag | C-Human Fc Tag |
| Molecular Characterization | IL1B(Ala117-Ser269) hFc(Glu99-Ala330) |
| Molecular Weight | The protein has a predicted molecular mass of 43.5 kDa after removal of the signal peptide.The apparent molecular mass of IL1B-hFc is approximately 35-55 kDa due to glycosylation. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2020] |
| Usage | Research use only |
Human IL1B Protein, hFc Tag
Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00
Product Data
图片
Figure 1. Human IL1B Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human IL1B Protein, hFc Tag (PME100838) can bind Anti-IL1B Neutralizing antibody (BME100116) in a linear range of 0.61–156.25 ng/mL.