缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。
ECD 蛋白（Extracellular Domain Proteins）
什么是ECD蛋白？
ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。
ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。
特色与应用
特色
哺乳动物细胞表达
天然构象及完整翻译后修饰
成功验证可做免疫原用于治疗性抗体开发
覆盖1000+药物靶点
针对同一靶点提供不同种属及不同片段蛋白
可用于种属交叉验证及抗体表位筛选
支持多种治疗性抗体功能筛选验证
SDS-PAGE | ELISA | Flow Cyt | SPR
免疫原应用数据
ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.
Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.
Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
蛋白活性数据
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.
Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.
Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
热门靶点分类
热点速递
产品列表
ECD Proteins
SKU: PME100806 Target: SSTR2 Tag: C-Human Fc Tag
Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00
ECD Proteins
SKU: PME100481 Target: CD30 Tag: C-6×His Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
ECD Proteins
SKU: PME100018 Target: GITR Tag: C-Human Fc and 6×His Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
ECD Proteins
SKU: PME100009 Target: SIRPa Tag: C-Human Fc and 6×His Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME44614 Target: Mrc2 Tag: C-MYC/DDK
Price: 20 μg ￥2900.00
ECD Proteins
SKU: PME101210 Target: AKT1 Tag: C-8×His Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME30080 Target: CCL16 Tag:
Price: 10 μg ￥936.00 ; 50 μg ￥2800.00
ECD Proteins
SKU: PME30045 Target: IL-29 Tag: C-10×His Tag
Price: 10 μg ￥600.00 ; 50 μg ￥1800.00
ECD Proteins
SKU: PME101097 Target: CD11 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101096 Target: LALBA Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101084 Target: FMNL1 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101082 Target: LILRA5 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101074 Target: GPC2 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101073 Target: CD1D Tag: C-Human Fc tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101072 Target: TIE2 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101071 Target: CD305 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101066 Target: ULBP2 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101061 Target: PPT Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101059 Target: DLK1 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101057 Target: TGFA Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101043 Target: RET Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101028 Target: TYRO3 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101026 Target: TNFRSF10A Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101016 Target: TGFB3 Tag: C-Human Fc tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100998 Target: PRAME Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100997 Target: POTED Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100996 Target: PCDH18 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100995 Target: PBF Tag: C-Human Fc Tag
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ECD Proteins
SKU: PME100986 Target: Myeloblastin Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100982 Target: MRP1 Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100971 Target: LAMP Tag: C-Human Fc Tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME100969 Target: KLK3 Tag: C-Human Fc tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
