ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-PME101393 DR6 Fig.1 SDS PAGE 1

ECD Proteins

Human DR6 Protein, His Tag

SKU:  PME101393     Target:  DR6     Tag:  C-6×His tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101392 FCN1 Fig.1 SDS PAGE 1

ECD Proteins

Human FCN1 Protein, His Tag

SKU:  PME101392     Target:  FCN1     Tag:  C-6×His tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101391 FCRL5 Fig.1 SDS PAGE 1

ECD Proteins

Human FCRL5(752-834) Protein, mFc Tag

SKU:  PME101391     Target:  FCRL5     Tag:  C-mouse Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101390 FCRL5 Fig.1 SDS PAGE 1

ECD Proteins

Human FCRL5(752-834) Protein, hFc Tag

SKU:  PME101390     Target:  FCRL5     Tag:  C-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101384 CDH17 Fig.1 SDS PAGE 1

ECD Proteins

Human CDH17(567-667) Protein, mFc Tag

SKU:  PME101384     Target:  CDH17     Tag:  C-mouse Fc tag

Price:10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101364 APO 1 Fig.1 SDS PAGE 1

ECD Proteins

Human TNFRSF6 Protein, hFc Tag

SKU:  PME101364     Target:  TNFRSF6     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101362 MMP8 Fig.1 SDS PAGE 1

ECD Proteins

Human MMP8 Protein, His Tag

SKU:  PME101362     Target:  MMP8     Tag:  C-6×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-PME101359 ST2 Fig.1 SDS PAGE 1

ECD Proteins

Human ST2 Protein, His Tag

SKU:  PME101359     Target:  ST2     Tag:  C-6×His tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101358 PDGFRA Fig.1 SDS PAGE 1

ECD Proteins

Human PDGFRA Protein, His Tag

SKU:  PME101358     Target:  PDGFRA     Tag:  C-6×His tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101389 CSPG4 Fig.1 SDS PAGE 1

ECD Proteins

Human CSPG4(1538-2221) Protein, hFc Tag

SKU:  PME101389     Target:  CSPG4     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101388 PRLR Fig.1 SDS PAGE 1

ECD Proteins

Human PRLR Protein, His Tag

SKU:  PME101388     Target:  PRLR     Tag:  C-10×His tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101387 CD28 Fig.1 SDS PAGE 1

ECD Proteins

Human CD28 Protein, His Tag

SKU:  PME101387     Target:  CD28     Tag:  C-10×His tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101386 ITGAVITGB6 Fig.1 SDS PAGE 1

ECD Proteins

Human ITGAV and ITGB6 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME101386     Target:  ITGAV and ITGB6     Tag:  C-6×His tag and C-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101385 CSPG4 Fig.1 SDS PAGE 1

ECD Proteins

Human CSPG4(1538-2221) Protein, mFc Tag

SKU:  PME101385     Target:  CSPG4     Tag:  C-mouse Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101380 CD22 Fig.1 SDS PAGE 1

ECD Proteins

Human CD22(417-678) Protein, mFc Tag

SKU:  PME101380     Target:  CD22     Tag:  C-mouse Fc tag

Price:10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101379 CD22 Fig.1 SDS PAGE 1

ECD Proteins

Human CD22(417-678) Protein, hFc Tag

SKU:  PME101379     Target:  CD22     Tag:  C-Human Fc tag

Price:10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101378 ITGB6 Fig.1 SDS PAGE 1

ECD Proteins

Human ITGB6 Protein, hFc Tag

SKU:  PME101378     Target:  ITGB6     Tag:  C-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101377 RAMP1 Fig.1 SDS PAGE 1

ECD Proteins

Human RAMP1 Protein, mFc Tag

SKU:  PME101377     Target:  RAMP1     Tag:  C-mouse Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101376 CGRPR Fig.1 SDS PAGE 1

ECD Proteins

Human CGRPR Protein, mFc Tag

SKU:  PME101376     Target:  CGRPR     Tag:  C-mouse Fc tag

Price:10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101375 CGRPR Fig.1 SDS PAGE 1

ECD Proteins

Human CGRPR Protein, hFc Tag

SKU:  PME101375     Target:  CGRPR     Tag:  C-Human Fc tag

Price:10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101373 CCN2 Fig.1 SDS PAGE 1

ECD Proteins

Human CCN2 Protein, hFc Tag

SKU:  PME101373     Target:  CCN2     Tag:  N-Human Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101372 GDF8 Fig.1 SDS PAGE 1

ECD Proteins

Human GDF8 Protein, hFc Tag

SKU:  PME101372     Target:  GDF8     Tag:  C-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101371 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(42-54) Protein, mFc Tag

SKU:  PME101371     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101370 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(22-54) Protein, mFc Tag

SKU:  PME101370     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101369 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(22-42) Protein, mFc Tag

SKU:  PME101369     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101368 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(1-42) Protein, mFc Tag

SKU:  PME101368     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101367 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(1-22) Protein, mFc Tag

SKU:  PME101367     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101366 LRP5 Fig.1 SDS PAGE 1

ECD Proteins

Human LRP5 Protein, hFc Tag

SKU:  PME101366     Target:  LRP5     Tag:  N-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101365 PRL Fig.1 SDS PAGE 1

ECD Proteins

Human PRL Protein, hFc Tag

SKU:  PME101365     Target:  PRL     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101363 IL3 Fig.1 SDS PAGE 1

ECD Proteins

Human IL3 Protein, hFc Tag

SKU:  PME101363     Target:  IL3     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101361 MADCAM1 Fig.1 SDS PAGE 1 1

ECD Proteins

Human MADCAM1 Protein, His Tag

SKU:  PME101361     Target:  MADCAM1     Tag:  C-6×His tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101360 OSCAR Fig.1 SDS PAGE 1

ECD Proteins

Human OSCAR Protein, His Tag

SKU:  PME101360     Target:  OSCAR     Tag:  C-6×His tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00