ECD 蛋白（Extracellular Domain Proteins）

ECD 蛋白（Extracellular Domain Proteins，胞外结构域蛋白）是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。靶点包括GPCR、免疫检查点、肿瘤相关抗原及代谢受体等多个热门可成药靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用，为膜蛋白药物研发提供高质量研究材料。

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

通过去除跨膜结构并优化表达体系，可获得可溶、正确折叠的ECD重组蛋白，既能保留关键抗原表位，又便于ELISA、SPR、流式检测、抗体筛选及结构分析等多种实验应用。随着结构生物学与蛋白工程技术的发展，ECD蛋白已广泛应用于抗体药物发现、受体-配体互作研究以及疫苗设计等领域。

ECD蛋白的应用

  • 抗体初筛（Primary Screening）
  • 抗体亲和力比较与排序
  • 表位分组与机制研究
  • ELISA/SPR/BLI方法建立
  • 全长膜蛋白或细胞实验前的验证步骤

缔码ECD蛋白优势

  • 哺乳动物细胞表达，构象更稳定，筛选更可靠
  • 多物种、多片段现货，快速匹配不同研究需求
  • 1600+现货ECD蛋白，覆盖900+药物靶点
  • 支持流式抗体开发，500+项目实际应用验证
  • 适配多种应用场景，加速抗体开发流程

ECD蛋白热门分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

ECD蛋白数据展示

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

The List of ECD Proteins

sp-PME101370 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(22-54) Protein, mFc Tag

SKU:  PME101370     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101369 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(22-42) Protein, mFc Tag

SKU:  PME101369     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101368 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(1-42) Protein, mFc Tag

SKU:  PME101368     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101367 BCMA Fig.1 SDS PAGE 1

ECD Proteins

Human BCMA(1-22) Protein, mFc Tag

SKU:  PME101367     Target:  BCMA     Tag:  C-mouse Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101366 LRP5 Fig.1 SDS PAGE 1

ECD Proteins

Human LRP5 Protein, hFc Tag

SKU:  PME101366     Target:  LRP5     Tag:  N-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101365 PRL Fig.1 SDS PAGE 1

ECD Proteins

Human PRL Protein, hFc Tag

SKU:  PME101365     Target:  PRL     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101363 IL3 Fig.1 SDS PAGE 1

ECD Proteins

Human IL3 Protein, hFc Tag

SKU:  PME101363     Target:  IL3     Tag:  C-Human Fc tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101361 MADCAM1 Fig.1 SDS PAGE 1 1

ECD Proteins

Human MADCAM1 Protein, His Tag

SKU:  PME101361     Target:  MADCAM1     Tag:  C-6×His tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101360 OSCAR Fig.1 SDS PAGE 1

ECD Proteins

Human OSCAR Protein, His Tag

SKU:  PME101360     Target:  OSCAR     Tag:  C-6×His tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME100358 MUC16 Fig.1 SDS PAGE 1

ECD Proteins

Human MUC16(13810-14451) Protein, His Tag

SKU:  PME100358     Target:  MUC16     Tag:  C-10×His tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101355 APLN Fig.1 SDS PAGE 1

ECD Proteins

Human APLN Protein, hFc Tag

SKU:  PME101355     Target:  APLN     Tag:  C-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101347 C5 Fig.1 SDS PAGE 1

ECD Proteins

Human C5 Protein, His Tag

SKU:  PME101347     Target:  C5     Tag:  C-10×His Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101345 LEPR Fig.1 SDS PAGE 1

ECD Proteins

Human LEPR Protein, His Tag

SKU:  PME101345     Target:  LEPR     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101343 CX3CL1 Fig.1 SDS PAGE 1 1

ECD Proteins

Human CX3CL1 Protein, His Tag

SKU:  PME101343     Target:  CX3CL1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101342 C2 Fig.1 SDS PAGE 1

ECD Proteins

Human C2 Protein, His Tag

SKU:  PME101342     Target:  C2     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101337 FGFR2 Fig.1 SDS PAGE 1 1

ECD Proteins

Human FGFR2c Protein, His Tag

SKU:  PME101337     Target:  FGFR2     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME100199 CDH17 Fig.1 SDS PAGE 1 1

ECD Proteins

Human CDH17(567-667) Protein, hFc Tag

SKU:  PME100199     Target:  CDH17     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101409 MET Fig.1 SDS PAGE 1

ECD Proteins

Human MET Protein, hFc Tag

SKU:  PME101409     Target:  MET     Tag:  C-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101357 MICAa3 Fig.1 SDS PAGE 1

ECD Proteins

Human MICAa3 Protein, His Tag

SKU:  PME101357     Target:  MICAa3     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101356 MICAa3 Fig.1 SDS PAGE 1

ECD Proteins

Human MICAa3 Protein, mFc Tag

SKU:  PME101356     Target:  MICAa3     Tag:  C-mouse Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101354 F2RL3 Fig.1 SDS PAGE 1

ECD Proteins

Human F2RL3 Protein, hFc Tag

SKU:  PME101354     Target:  F2RL3     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101353 GPR132 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR132 Protein, hFc Tag

SKU:  PME101353     Target:  GPR132     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101352 IL25 Fig.1 SDS PAGE 1

ECD Proteins

Human IL25 Protein, hFc Tag

SKU:  PME101352     Target:  IL25     Tag:  C-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101351 CD62E Fig.1 SDS PAGE 1

ECD Proteins

Human CD62E Protein, His Tag

SKU:  PME101351     Target:  CD62E     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101350 CD161 Fig.1 SDS PAGE 1

ECD Proteins

Human CD161 Protein, hFc Tag

SKU:  PME101350     Target:  CD161     Tag:  N-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101349 TREML1 Fig.1 SDS PAGE 1

ECD Proteins

Human TREML1 Protein, hFc Tag

SKU:  PME101349     Target:  TREML1     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101348 CFH Fig.1 SDS PAGE 1

ECD Proteins

Human CFH Protein, His Tag

SKU:  PME101348     Target:  CFH     Tag:  C-10×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101346 TREML2 Fig.1 SDS PAGE 1

ECD Proteins

Human TREML2 Protein, His Tag

SKU:  PME101346     Target:  TREML2     Tag:  C-6×His Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101344 APOH Fig.1 SDS PAGE 1

ECD Proteins

Human APOH Protein, His Tag

SKU:  PME101344     Target:  APOH     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101341 MAG Fig.1 SDS PAGE 1 1

ECD Proteins

Human MAG Protein, His Tag

SKU:  PME101341     Target:  MAG     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101340 MFAP4 Fig.1 SDS PAGE 1 1

ECD Proteins

Human MFAP4 Protein, His Tag

SKU:  PME101340     Target:  MFAP4     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101339 AOC3 Fig.1 SDS PAGE 1 1

ECD Proteins

Human AOC3 Protein, His Tag

SKU:  PME101339     Target:  AOC3     Tag:  N-6×His Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00