ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

人、鼠、猴、犬源重组蛋白：抗体药物研发的隐藏关键

在抗体药物（therapeutic antibody）研发流程中，…

14
11 月
靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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12 月

产品列表

sp-PME101329 WFDC1 Fig.1 SDS PAGE 1

ECD Proteins

Human WFDC1 Protein, hFc Tag

SKU:  PME101329     Target:  WFDC1     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101328 MXRA8 Fig.1 SDS PAGE 1

ECD Proteins

Human MXRA8 Protein, His Tag

SKU:  PME101328     Target:  MXRA8     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101327 LILRB5 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRB5 Protein, His Tag

SKU:  PME101327     Target:  LILRB5     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101326 LILRA2 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRA2 Protein, His Tag

SKU:  PME101326     Target:  LILRA2     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101325 IL13RA2 Fig.1 SDS PAGE 1

ECD Proteins

Human IL13RA2 Protein, hFc Tag

SKU:  PME101325     Target:  IL13RA2     Tag:  C-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101324 VWF Fig.1 SDS PAGE 1

ECD Proteins

Human VWF(1596-1668) Protein, hFc Tag

SKU:  PME101324     Target:  VWF     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101323 GPR65 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR65 Protein, hFc Tag

SKU:  PME101323     Target:  GPR65     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101322 CD79A Fig.1 SDS PAGE 1

ECD Proteins

Human CD79A Protein, hFc Tag

SKU:  PME101322     Target:  CD79A     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101321 EGFRVIII Fig.1 SDS PAGE 1

ECD Proteins

Human EGFRVIII Protein, His Tag

SKU:  PME101321     Target:  EGFRVIII     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101320 ENPP2 Fig.1 SDS PAGE 1

ECD Proteins

Human ENPP2 Protein, His Tag

SKU:  PME101320     Target:  ENPP2     Tag:  N-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101319 CSPG5 Fig.1 SDS PAGE 1

ECD Proteins

Human CSPG5 Protein, His Tag

SKU:  PME101319     Target:  CSPG5     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101317 SEMA7A Fig.1 SDS PAGE 1

ECD Proteins

Human SEMA7A Protein, His Tag

SKU:  PME101317     Target:  SEMA7A     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101316 PLAT Fig.1 SDS PAGE 1

ECD Proteins

Human PLAT Protein, His Tag

SKU:  PME101316     Target:  PLAT     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101315 CCL11 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL11 Protein, hFc Tag

SKU:  PME101315     Target:  CCL11     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101314 CFD Fig.1 SDS PAGE 1

ECD Proteins

Human CFD Protein, hFc Tag

SKU:  PME101314     Target:  CFD     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101313 CLEC5A Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC5A Protein, hFc Tag

SKU:  PME101313     Target:  CLEC5A     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101312 LOX 1 Fig.1 SDS PAGE 1

ECD Proteins

Human LOX-1 Protein, hFc Tag

SKU:  PME101312     Target:  LOX-1     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101311 LILRB4 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRB4 Protein, hFc Tag

SKU:  PME101311     Target:  LILRB4     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101310 CLEC4C Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC4C Protein, His Tag

SKU:  PME101310     Target:  CLEC4C     Tag:  C-6×His Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101308 IGSF11 Fig.1 SDS PAGE 1

ECD Proteins

Human IGSF11 Protein, hFc Tag

SKU:  PME101308     Target:  IGSF11     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101307 IL4 Fig.1 SDS PAGE 1

ECD Proteins

Human IL4 Protein, mFc Tag

SKU:  PME101307     Target:  IL4     Tag:  C-mouse Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101304 PAI1 Fig.1 SDS PAGE 1

ECD Proteins

Human PAI1 Protein, His Tag

SKU:  PME101304     Target:  PAI1     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101303 MELTF Fig.1 SDS PAGE 1

ECD Proteins

Human MELTF Protein, His Tag

SKU:  PME101303     Target:  MELTF     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101292 GPR64 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR64 Protein, His Tag

SKU:  PME101292     Target:  GPR64     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME100319 KIR2DL3 Fig.1 SDS PAGE 1

ECD Proteins

Human KIR2DL3 Protein, His Tag

SKU:  PME100319     Target:  KIR2DL3     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

elisa-PME30021 C687 P08476 ELISA 0

ECD Proteins

Human/Mouse/Rat Activin A Protein

SKU:  PME30021     Target:  Activin     Tag: 

Price: 10 μg ￥1200.00 ; 50 μg ￥3520.00

sp-PME101400 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-164) Protein, hFc Tag

SKU:  PME101400     Target:  PLA2R1     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101399 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-521+1097-1246) Protein, His Tag

SKU:  PME101399     Target:  PLA2R1     Tag:  C-10×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME C100016 SELPLG Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus SELPLG Protein, His Tag

SKU:  PME-C100016     Target:  SELPLG     Tag:  C-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-PME C100015 IL18BP Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus IL18BP Protein, His Tag

SKU:  PME-C100015     Target:  IL18BP     Tag:  C-6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-PME C100014 CD93 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD93 Protein, His Tag

SKU:  PME-C100014     Target:  CD93     Tag:  C-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-PME C100013 CLEC4C Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CLEC4C Protein, His Tag

SKU:  PME-C100013     Target:  CLEC4C     Tag:  N-6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00