ECD 蛋白（Extracellular Domain Proteins）

ECD 蛋白（Extracellular Domain Proteins，胞外结构域蛋白）是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。靶点包括GPCR、免疫检查点、肿瘤相关抗原及代谢受体等多个热门可成药靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用，为膜蛋白药物研发提供高质量研究材料。

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

通过去除跨膜结构并优化表达体系，可获得可溶、正确折叠的ECD重组蛋白，既能保留关键抗原表位，又便于ELISA、SPR、流式检测、抗体筛选及结构分析等多种实验应用。随着结构生物学与蛋白工程技术的发展，ECD蛋白已广泛应用于抗体药物发现、受体-配体互作研究以及疫苗设计等领域。

ECD蛋白的应用

  • 抗体初筛（Primary Screening）
  • 抗体亲和力比较与排序
  • 表位分组与机制研究
  • ELISA/SPR/BLI方法建立
  • 全长膜蛋白或细胞实验前的验证步骤

缔码ECD蛋白优势

  • 哺乳动物细胞表达，构象更稳定，筛选更可靠
  • 多物种、多片段现货，快速匹配不同研究需求
  • 1600+现货ECD蛋白，覆盖900+药物靶点
  • 支持流式抗体开发，500+项目实际应用验证
  • 适配多种应用场景，加速抗体开发流程

ECD蛋白热门分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

ECD蛋白数据展示

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

The List of ECD Proteins

sp-PME101317 SEMA7A Fig.1 SDS PAGE 1

ECD Proteins

Human SEMA7A Protein, His Tag

SKU:  PME101317     Target:  SEMA7A     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101316 PLAT Fig.1 SDS PAGE 1

ECD Proteins

Human PLAT Protein, His Tag

SKU:  PME101316     Target:  PLAT     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101315 CCL11 Fig.1 SDS PAGE 1

ECD Proteins

Human CCL11 Protein, hFc Tag

SKU:  PME101315     Target:  CCL11     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101314 CFD Fig.1 SDS PAGE 1

ECD Proteins

Human CFD Protein, hFc Tag

SKU:  PME101314     Target:  CFD     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101313 CLEC5A Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC5A Protein, hFc Tag

SKU:  PME101313     Target:  CLEC5A     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101312 LOX 1 Fig.1 SDS PAGE 1

ECD Proteins

Human LOX-1 Protein, hFc Tag

SKU:  PME101312     Target:  LOX-1     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101311 LILRB4 Fig.1 SDS PAGE 1

ECD Proteins

Human LILRB4 Protein, hFc Tag

SKU:  PME101311     Target:  LILRB4     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101310 CLEC4C Fig.1 SDS PAGE 1

ECD Proteins

Human CLEC4C Protein, His Tag

SKU:  PME101310     Target:  CLEC4C     Tag:  C-6×His Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101308 IGSF11 Fig.1 SDS PAGE 1

ECD Proteins

Human IGSF11 Protein, hFc Tag

SKU:  PME101308     Target:  IGSF11     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101307 IL4 Fig.1 SDS PAGE 1

ECD Proteins

Human IL4 Protein, mFc Tag

SKU:  PME101307     Target:  IL4     Tag:  C-mouse Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101304 PAI1 Fig.1 SDS PAGE 1

ECD Proteins

Human PAI1 Protein, His Tag

SKU:  PME101304     Target:  PAI1     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101303 MELTF Fig.1 SDS PAGE 1

ECD Proteins

Human MELTF Protein, His Tag

SKU:  PME101303     Target:  MELTF     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101292 GPR64 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR64 Protein, His Tag

SKU:  PME101292     Target:  GPR64     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME100319 KIR2DL3 Fig.1 SDS PAGE 1

ECD Proteins

Human KIR2DL3 Protein, His Tag

SKU:  PME100319     Target:  KIR2DL3     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

elisa-PME30021 C687 P08476 ELISA 0

ECD Proteins

Human/Mouse/Rat Activin A Protein

SKU:  PME30021     Target:  Activin     Tag: 

Price: 10 μg ￥1200.00 ; 50 μg ￥3520.00

sp-PME101400 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-164) Protein, hFc Tag

SKU:  PME101400     Target:  PLA2R1     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101399 PLA2R1 Fig.1 SDS PAGE 1

ECD Proteins

Human PLA2R1(21-521+1097-1246) Protein, His Tag

SKU:  PME101399     Target:  PLA2R1     Tag:  C-10×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME C100016 SELPLG Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus SELPLG Protein, His Tag

SKU:  PME-C100016     Target:  SELPLG     Tag:  C-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-PME C100015 IL18BP Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus IL18BP Protein, His Tag

SKU:  PME-C100015     Target:  IL18BP     Tag:  C-6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-PME C100014 CD93 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CD93 Protein, His Tag

SKU:  PME-C100014     Target:  CD93     Tag:  C-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-PME C100013 CLEC4C Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus CLEC4C Protein, His Tag

SKU:  PME-C100013     Target:  CLEC4C     Tag:  N-6×His Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-PME C100012 ADAM9 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus ADAM9 Protein, His Tag

SKU:  PME-C100012     Target:  ADAM9     Tag:  C-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-PME101302 GPR81 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR81 Protein, hFc Tag

SKU:  PME101302     Target:  GPR81     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101301 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(110-139) Protein, hFc Tag

SKU:  PME101301     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101300 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(77-106) Protein, hFc Tag

SKU:  PME101300     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101299 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(44-72) Protein, hFc Tag

SKU:  PME101299     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101298 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(11-40) Protein, hFc Tag

SKU:  PME101298     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101297 IBSP Fig.1 SDS PAGE 1

ECD Proteins

Human IBSP Protein, His Tag

SKU:  PME101297     Target:  IBSP     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101296 IL4 Fig.1 SDS PAGE 1

ECD Proteins

Human IL4 Protein, His Tag

SKU:  PME101296     Target:  IL4     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101295 ARTN Fig.1 SDS PAGE 1

ECD Proteins

Human ARTN Protein, hFc Tag

SKU:  PME101295     Target:  ARTN     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101294 S100A9 Fig.1 SDS PAGE 1

ECD Proteins

Human S100A9 Protein, hFc Tag

SKU:  PME101294     Target:  S100A9     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101291 FSTL1 Fig.1 SDS PAGE 1

ECD Proteins

Human FSTL1 Protein, His Tag

SKU:  PME101291     Target:  FSTL1     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00