ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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产品列表

sp-pme101473 dll4 sp1

ECD Proteins

Human DLL4 Protein, His Tag

SKU:  PME101473     Target:  DLL4     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101472 tpo sp1

ECD Proteins

Human TPO Protein, His Tag

SKU:  PME101472     Target:  TPO     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101471 aplnr sp1

ECD Proteins

Human APLNR Protein, hFc Tag

SKU:  PME101471     Target:  APLNR     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101470 gpr84 sp1

ECD Proteins

Human GPR84 Protein, hFc Tag

SKU:  PME101470     Target:  GPR84     Tag:  C-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101465 cxcl14 sp1

ECD Proteins

Human CXCL14 Protein, hFc Tag

SKU:  PME101465     Target:  CXCL14     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101464 ccl19 sp1

ECD Proteins

Human CCL19 Protein, hFc Tag

SKU:  PME101464     Target:  CCL19     Tag:  N-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101463 f9 sp1

ECD Proteins

Human F9 Protein, His Tag

SKU:  PME101463     Target:  F9     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101462 f10 sp1

ECD Proteins

Human F10 Protein, His Tag

SKU:  PME101462     Target:  F10     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101460 ntsr1 sp1

ECD Proteins

Human NTSR1 Protein, hFc Tag

SKU:  PME101460     Target:  NTSR1     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101459 clec10a sp1

ECD Proteins

Human CLEC10A Protein, hFc Tag

SKU:  PME101459     Target:  CLEC10A     Tag:  N-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101458 uts2r sp1

ECD Proteins

Human UTS2R Protein, hFc Tag

SKU:  PME101458     Target:  UTS2R     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101457 fshb sp1

ECD Proteins

Human FSHB Protein, hFc Tag

SKU:  PME101457     Target:  FSHB     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101456 ngal sp1

ECD Proteins

Human NGAL Protein, hFc Tag

SKU:  PME101456     Target:  NGAL     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101455 npy sp1 1

ECD Proteins

Human NPY Protein, hFc Tag

SKU:  PME101455     Target:  NPY     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101454 gip sp1 1

ECD Proteins

Human GIP Protein, hFc Tag

SKU:  PME101454     Target:  GIP     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101453 hmgb1 sp1 1

ECD Proteins

Human HMGB1 Protein, His Tag

SKU:  PME101453     Target:  HMGB1     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101452 c6 sp1 1

ECD Proteins

Human C6 Protein, His Tag

SKU:  PME101452     Target:  C6     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101451 cadm3 sp1 1

ECD Proteins

Human CADM3 Protein, His Tag

SKU:  PME101451     Target:  CADM3     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101450 ephb3 sp1 1

ECD Proteins

Human EPHB3 Protein, His Tag

SKU:  PME101450     Target:  EPHB3     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101449 pomc sp1 1

ECD Proteins

Human POMC Protein, His Tag

SKU:  PME101449     Target:  POMC     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101448 mog sp1 1

ECD Proteins

Human MOG Protein, hFc Tag

SKU:  PME101448     Target:  MOG     Tag:  C-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101446 ccl24 sp1 1

ECD Proteins

Human CCL24 Protein, hFc Tag

SKU:  PME101446     Target:  CCL24     Tag:  C-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101441 mapt sp1

ECD Proteins

Human MAPT Protein, His Tag

SKU:  PME101441     Target:  MAPT     Tag:  N-6×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme101430 ttr sp1

ECD Proteins

Human TTR Protein, hFc Tag

SKU:  PME101430     Target:  TTR     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme f100001 il31 sp1

ECD Proteins

Feline IL31 Protein, His Tag

SKU:  PME-F100001     Target:  IL31     Tag:  C-6×His tag

Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00

sp-pme d100004 il31 sp1

ECD Proteins

Canine IL31 Protein, His Tag

SKU:  PME-D100004     Target:  IL31     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101444 ror1 sp1

ECD Proteins

Human ROR1(838-937) Protein, hFc Tag

SKU:  PME101444     Target:  ROR1     Tag:  N-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101443 her3 sp1

ECD Proteins

Human HER3(1243-1342) Protein, hFc Tag

SKU:  PME101443     Target:  HER3     Tag:  N-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101442 tim3 sp1

ECD Proteins

Human TIM3 Protein, hFc Tag

SKU:  PME101442     Target:  TIM3     Tag:  C-Human Fc tag

Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00

sp-pme101440 pedf sp1

ECD Proteins

Human PEDF Protein, His Tag

SKU:  PME101440     Target:  PEDF     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101439 apoe sp1

ECD Proteins

Human APOE Protein, His Tag

SKU:  PME101439     Target:  APOE     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101438 c9 sp1

ECD Proteins

Human C9 Protein, His Tag

SKU:  PME101438     Target:  C9     Tag:  C-6×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00