ECD 蛋白（Extracellular Domain Proteins）

ECD 蛋白（Extracellular Domain Proteins，胞外结构域蛋白）是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。靶点包括GPCR、免疫检查点、肿瘤相关抗原及代谢受体等多个热门可成药靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用，为膜蛋白药物研发提供高质量研究材料。

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

通过去除跨膜结构并优化表达体系，可获得可溶、正确折叠的ECD重组蛋白，既能保留关键抗原表位，又便于ELISA、SPR、流式检测、抗体筛选及结构分析等多种实验应用。随着结构生物学与蛋白工程技术的发展，ECD蛋白已广泛应用于抗体药物发现、受体-配体互作研究以及疫苗设计等领域。

ECD蛋白的应用

  • 抗体初筛（Primary Screening）
  • 抗体亲和力比较与排序
  • 表位分组与机制研究
  • ELISA/SPR/BLI方法建立
  • 全长膜蛋白或细胞实验前的验证步骤

缔码ECD蛋白优势

  • 哺乳动物细胞表达，构象更稳定，筛选更可靠
  • 多物种、多片段现货，快速匹配不同研究需求
  • 1600+现货ECD蛋白，覆盖900+药物靶点
  • 支持流式抗体开发，500+项目实际应用验证
  • 适配多种应用场景，加速抗体开发流程

ECD蛋白热门分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

ECD蛋白数据展示

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

The List of ECD Proteins

sp-pme d100010 il31ra sp1

ECD Proteins

Canine IL31RA Protein, mFc Tag

SKU:  PME-D100010     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme c100060 bcma sp1

ECD Proteins

Cynomolgus BCMA Protein, hFc Tag

SKU:  PME-C100060     Target:  BCMA     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme c100059 ror2 sp1

ECD Proteins

Cynomolgus ROR2 Protein, N-His Tag

SKU:  PME-C100059     Target:  ROR2     Tag:  N-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100058 ror2 sp1

ECD Proteins

Cynomolgus ROR2 Protein, C-His Tag

SKU:  PME-C100058     Target:  ROR2     Tag:  C-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100057 trop2 sp1

ECD Proteins

Cynomolgus Trop2 Protein, His Tag

SKU:  PME-C100057     Target:  Trop2     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100112 cdh6 sp1

ECD Proteins

Mouse CDH6 Protein, His Tag

SKU:  PME-M100112     Target:  CDH6     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101500 il1ra sp1

ECD Proteins

Human IL1RA Protein, mFc Tag

SKU:  PME101500     Target:  IL1RA     Tag:  C-mouse Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101475 folr3 sp1

ECD Proteins

Human FOLR3 Protein, His Tag

SKU:  PME101475     Target:  FOLR3     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101474 efnb1 sp1

ECD Proteins

Human EFNB1 Protein, His Tag

SKU:  PME101474     Target:  EFNB1     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101473 dll4 sp1

ECD Proteins

Human DLL4 Protein, His Tag

SKU:  PME101473     Target:  DLL4     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101472 tpo sp1

ECD Proteins

Human TPO Protein, His Tag

SKU:  PME101472     Target:  TPO     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101471 aplnr sp1

ECD Proteins

Human APLNR Protein, hFc Tag

SKU:  PME101471     Target:  APLNR     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101470 gpr84 sp1

ECD Proteins

Human GPR84 Protein, hFc Tag

SKU:  PME101470     Target:  GPR84     Tag:  C-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101465 cxcl14 sp1

ECD Proteins

Human CXCL14 Protein, hFc Tag

SKU:  PME101465     Target:  CXCL14     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101464 ccl19 sp1

ECD Proteins

Human CCL19 Protein, hFc Tag

SKU:  PME101464     Target:  CCL19     Tag:  N-Human Fc tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101463 f9 sp1

ECD Proteins

Human F9 Protein, His Tag

SKU:  PME101463     Target:  F9     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101462 f10 sp1

ECD Proteins

Human F10 Protein, His Tag

SKU:  PME101462     Target:  F10     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101460 ntsr1 sp1

ECD Proteins

Human NTSR1 Protein, hFc Tag

SKU:  PME101460     Target:  NTSR1     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101459 clec10a sp1

ECD Proteins

Human CLEC10A Protein, hFc Tag

SKU:  PME101459     Target:  CLEC10A     Tag:  N-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101458 uts2r sp1

ECD Proteins

Human UTS2R Protein, hFc Tag

SKU:  PME101458     Target:  UTS2R     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101457 fshb sp1

ECD Proteins

Human FSHB Protein, hFc Tag

SKU:  PME101457     Target:  FSHB     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101456 ngal sp1

ECD Proteins

Human NGAL Protein, hFc Tag

SKU:  PME101456     Target:  NGAL     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101455 npy sp1 1

ECD Proteins

Human NPY Protein, hFc Tag

SKU:  PME101455     Target:  NPY     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101454 gip sp1 1

ECD Proteins

Human GIP Protein, hFc Tag

SKU:  PME101454     Target:  GIP     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101453 hmgb1 sp1 1

ECD Proteins

Human HMGB1 Protein, His Tag

SKU:  PME101453     Target:  HMGB1     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101452 c6 sp1 1

ECD Proteins

Human C6 Protein, His Tag

SKU:  PME101452     Target:  C6     Tag:  C-6×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101451 cadm3 sp1 1

ECD Proteins

Human CADM3 Protein, His Tag

SKU:  PME101451     Target:  CADM3     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101450 ephb3 sp1 1

ECD Proteins

Human EPHB3 Protein, His Tag

SKU:  PME101450     Target:  EPHB3     Tag:  C-6×His tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101449 pomc sp1 1

ECD Proteins

Human POMC Protein, His Tag

SKU:  PME101449     Target:  POMC     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101448 mog sp1 1

ECD Proteins

Human MOG Protein, hFc Tag

SKU:  PME101448     Target:  MOG     Tag:  C-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101446 ccl24 sp1 1

ECD Proteins

Human CCL24 Protein, hFc Tag

SKU:  PME101446     Target:  CCL24     Tag:  C-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101441 mapt sp1

ECD Proteins

Human MAPT Protein, His Tag

SKU:  PME101441     Target:  MAPT     Tag:  N-6×His tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00