ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

人、鼠、猴、犬源重组蛋白：抗体药物研发的隐藏关键

在抗体药物（therapeutic antibody）研发流程中，…

14
11 月
靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

04
12 月

产品列表

sp-pme101467 itgav itgb5 sp1

ECD Proteins

Human ITGAV and ITGB5 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME101467     Target:  ITGAV and ITGB5     Tag:  C-6×His tag and C-Human Fc tag

Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00

sp-pme c100066 dll3 sp1

ECD Proteins

Cynomolgus DLL3(350-488) Protein, hFc Tag

SKU:  PME-C100066     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100065 dll3 sp1

ECD Proteins

Cynomolgus DLL3(426-488) Protein, hFc Tag

SKU:  PME-C100065     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100064 dll3 sp1

ECD Proteins

Cynomolgus DLL3(388-488) Protein, hFc Tag

SKU:  PME-C100064     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100063 dll3 sp1

ECD Proteins

Cynomolgus DLL3(309-488) Protein, hFc Tag

SKU:  PME-C100063     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100062 dll3 sp1 1

ECD Proteins

Cynomolgus DLL3(272-488) Protein, His Tag

SKU:  PME-C100062     Target:  DLL3     Tag:  C-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100061 fcrl5 sp1 1

ECD Proteins

Cynomolgus FCRL5 Protein, His Tag

SKU:  PME-C100061     Target:  FCRL5     Tag:  C-10×His tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme m100114 folr1 sp1

ECD Proteins

Mouse FOLR1 Protein, His Tag

SKU:  PME-M100114     Target:  FOLR1     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme m100113 fcrl5 sp1

ECD Proteins

Mouse FCRL5 Protein, His Tag

SKU:  PME-M100113     Target:  FCRL5     Tag:  C-10×His tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme101532 dll3 sp1 1

ECD Proteins

Human DLL3(176-215) Protein, hFc Tag

SKU:  PME101532     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101505 dll3 sp1 1

ECD Proteins

Human DLL3(428-492) Protein, hFc Tag

SKU:  PME101505     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101504 dll3 sp1 1

ECD Proteins

Human DLL3(311-492) Protein, hFc Tag

SKU:  PME101504     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101499 dll3 sp1 1

ECD Proteins

Human DLL3(390-492) Protein, hFc Tag

SKU:  PME101499     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101498 dll3 sp1 1

ECD Proteins

Human DLL3(352-492) Protein, hFc Tag

SKU:  PME101498     Target:  DLL3     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101491 vegf165 sp1 1

ECD Proteins

Human VEGF165 Protein, hFc Tag

SKU:  PME101491     Target:  VEGF165     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme101490 vegf121 sp1 1

ECD Proteins

Human VEGF121 Protein, hFc Tag

SKU:  PME101490     Target:  VEGF121     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00

sp-pme100742 adamts13 sp1

ECD Proteins

Human ADAMTS13 Protein, His Tag

SKU:  PME100742     Target:  ADAMTS13     Tag:  C-6×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100026 muc1 sp1

ECD Proteins

Cynomolgus MUC1(254-373) Protein, His Tag

SKU:  PME-C100026     Target:  MUC1     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101528 hgf sp1

ECD Proteins

Human HGF(1-728) Protein, hFc Tag

SKU:  PME101528     Target:  HGF     Tag:  C-Human Fc tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101476 cd300c sp1

ECD Proteins

Human CD300C Protein, hFc Tag

SKU:  PME101476     Target:  CD300C     Tag:  C-Human Fc tag

Price:10μg ￥600.00; 50μg ￥1690.00; 100μg ￥2362.50

sp-pme101461 ccl17 sp1

ECD Proteins

Human CCL17 Protein, hFc Tag

SKU:  PME101461     Target:  CCL17     Tag:  C-Human Fc tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101445 cxcl10 sp1 1

ECD Proteins

Human CXCL10 Protein, hFc Tag

SKU:  PME101445     Target:  CXCL10     Tag:  N-Human Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme f100002 il31ra sp1

ECD Proteins

Feline IL31RA Protein, mFc Tag

SKU:  PME-F100002     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme d100010 il31ra sp1

ECD Proteins

Canine IL31RA Protein, mFc Tag

SKU:  PME-D100010     Target:  IL31RA     Tag:  C-Mouse Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme c100060 bcma sp1

ECD Proteins

Cynomolgus BCMA Protein, hFc Tag

SKU:  PME-C100060     Target:  BCMA     Tag:  C-Human Fc tag

Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-pme c100059 ror2 sp1

ECD Proteins

Cynomolgus ROR2 Protein, N-His Tag

SKU:  PME-C100059     Target:  ROR2     Tag:  N-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100058 ror2 sp1

ECD Proteins

Cynomolgus ROR2 Protein, C-His Tag

SKU:  PME-C100058     Target:  ROR2     Tag:  C-10×His tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100057 trop2 sp1

ECD Proteins

Cynomolgus Trop2 Protein, His Tag

SKU:  PME-C100057     Target:  Trop2     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100112 cdh6 sp1

ECD Proteins

Mouse CDH6 Protein, His Tag

SKU:  PME-M100112     Target:  CDH6     Tag:  C-10×His tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101500 il1ra sp1

ECD Proteins

Human IL1RA Protein, mFc Tag

SKU:  PME101500     Target:  IL1RA     Tag:  C-mouse Fc tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme101475 folr3 sp1

ECD Proteins

Human FOLR3 Protein, His Tag

SKU:  PME101475     Target:  FOLR3     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00

sp-pme101474 efnb1 sp1

ECD Proteins

Human EFNB1 Protein, His Tag

SKU:  PME101474     Target:  EFNB1     Tag:  C-6×His tag

Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100 μg ￥3160.00