ECD 蛋白（Extracellular Domain Proteins，胞外结构域蛋白）是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。靶点包括GPCR、免疫检查点、肿瘤相关抗原及代谢受体等多个热门可成药靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用，为膜蛋白药物研发提供高质量研究材料。
ECD 蛋白（Extracellular Domain Proteins）
什么是ECD蛋白？
ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。
通过去除跨膜结构并优化表达体系，可获得可溶、正确折叠的ECD重组蛋白，既能保留关键抗原表位，又便于ELISA、SPR、流式检测、抗体筛选及结构分析等多种实验应用。随着结构生物学与蛋白工程技术的发展，ECD蛋白已广泛应用于抗体药物发现、受体-配体互作研究以及疫苗设计等领域。
ECD蛋白的应用
- 抗体初筛（Primary Screening）
- 抗体亲和力比较与排序
- 表位分组与机制研究
- ELISA/SPR/BLI方法建立
- 全长膜蛋白或细胞实验前的验证步骤
缔码ECD蛋白优势
- 哺乳动物细胞表达，构象更稳定，筛选更可靠
- 多物种、多片段现货，快速匹配不同研究需求
- 1600+现货ECD蛋白，覆盖900+药物靶点
- 支持流式抗体开发，500+项目实际应用验证
- 适配多种应用场景，加速抗体开发流程
ECD蛋白热门分类
ECD蛋白数据展示
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.
Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.
Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
The List of ECD Proteins
ECD Proteins
SKU: PME101552 Target: RNF133 Tag: C-Human Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101567 Target: CDH17 Tag: C-Human Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101566 Target: CDH17 Tag: C-Human Fc tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101565 Target: CDH17 Tag: C-Human Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101564 Target: CDH17 Tag: C-Human Fc tag
Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00
ECD Proteins
SKU: PME101563 Target: CDH17 Tag: C-Human Fc tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101562 Target: CDH17 Tag: C-Human Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME-C100070 Target: MUC1 Tag: C-Mouse Fc tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00
ECD Proteins
SKU: PME-C100069 Target: MUC1 Tag: C-Human Fc tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00
ECD Proteins
SKU: PME-C100068 Target: MUC1 Tag: C-10×His tag
Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00
ECD Proteins
SKU: PME-C100067 Target: CRTAM Tag: C-10×His tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101551 Target: ACVR2B Tag: C-Human Fc tag
Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00
ECD Proteins
SKU: PME101550 Target: ACVR2A Tag: C-Human Fc tag
Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00
ECD Proteins
SKU: PME101549 Target: APOE2 Tag: C-6×His tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101548 Target: APOE4 Tag: C-6×His tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101547 Target: CRTAM Tag: C-mouse Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101546 Target: CRTAM Tag: C-Human Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101545 Target: CADM1 Tag: C-mouse Fc tag
Price: 10μg ￥706.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101544 Target: CADM1 Tag: C-Human Fc tag
Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00
ECD Proteins
SKU: PME101543 Target: MMP9 Tag: C-Human Fc tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME101542 Target: DLL1 Tag: C-6×His tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101541 Target: KIR2DL1 Tag: C-10×His tag
Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00
ECD Proteins
SKU: PME101540 Target: CLEC1A Tag: C-10×His tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101539 Target: LY6E Tag: C-Human Fc tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101538 Target: FGF19 Tag: C-Human Fc tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101537 Target: TNFSF15 Tag: C-10×His tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
ECD Proteins
SKU: PME101536 Target: ALPL Tag: C-10×His tag
Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00
ECD Proteins
SKU: PME101535 Target: CD3E Tag: C-10×His tag
Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00
ECD Proteins
SKU: PME101534 Target: ZNRF3 Tag: N-Human Fc tag
Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00
ECD Proteins
SKU: PME101533 Target: ADAMTS13 Tag: C-3×Flag Tag
Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00
ECD Proteins
SKU: PME101531 Target: ALPI Tag: C-10×His tag
Price: 10μg ￥630.00 ; 50μg ￥2250.00 ; 100μg ￥3160.00
ECD Proteins
SKU: PME101530 Target: STEAP1 Tag: N-Human Fc tag
Price: 10μg ￥668.00 ; 50μg ￥2300.00 ; 100μg ￥3499.00
