Recombinant human B7-2 protein with C-terminal human Fc tag
C-Human Fc Tag
The protein has a predicted molecular mass of 51.5 kDa after removal of the signal peptide.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.
Research use only
Human B7-2 Protein, hFc Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
Figure 1. Human B7-2 Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2, hFc tagged protein (PME100474) can bind Human CTLA4, mFc-His tagged protein (PME100017) in a linear range of 0.048-2.694 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2, hFc tagged protein (PME100474) can bind Human CD28, mFc-His tagged protein (PME100014) in a linear range of 62.5-4000 ng/ml.
Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human CD28 (A) were surface stained with Human B7-2 , hFc tagged protein (PME100474) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.