Recombinant Human BAFF protein with N-terminal human Fc
N-Human Fc Tag
The protein has a predicted molecular mass of 43.2 kDa after removal of the signal peptide.The apparent molecular mass of hFc-BAFF is approximately 50-55 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Research use only
Human BAFF Protein, hFc Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
Figure 1. Human BAFF Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BAFF, hFc tagged protein (PME100043) can bind Human BCMA, mFc tagged protein (PME100035) in a linear range of 0.03-15.625 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BAFF, hFc tagged protein (PME100043) can bind Human BAFF-R, mFc tagged protein (PME100044) in a linear range of 0.488-250.0 ng/ml.
Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BAFF, hFc tagged protein (PME100043) can bind Anti-BAFF Neutralizing antibody (BME100044) in a linear range of 3.2-400 ng/ml.
Figure 5. Flow cytometry analysis with 15 μg/mL Human BAFF Protein, hFc tag (PME100043) on Expi293 cells transfected with human BAFF-R (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).