ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-pme100666 gfap sp1

ECD Proteins

Human GFAP (7-66) Protein, hFc Tag

SKU:  PME100666     Target:  GFAP     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100665 her2 sp1

ECD Proteins

Human Her2 Protein, hFc Tag

SKU:  PME100665     Target:  Her2     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100664 kcnk9 sp1

ECD Proteins

Human KCNK9 Protein, hFc Tag

SKU:  PME100664     Target:  KCNK9     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100663 slc2a4 sp1

ECD Proteins

Human SLC2A4 Protein, hFc Tag

SKU:  PME100663     Target:  SLC2A4     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100662 gpr87 sp1

ECD Proteins

Human GPR87 Protein, hFc Tag

SKU:  PME100662     Target:  GPR87     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100660 ccr6 sp1

ECD Proteins

Human CCR6 Protein, hFc Tag

SKU:  PME100660     Target:  CCR6     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100659 nefl sp1

ECD Proteins

Human NEFL Protein, hFc Tag

SKU:  PME100659     Target:  NEFL     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100687 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Omicron) S protein RBD, hFc Tag

SKU:  PME100687     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100658 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Delta) S protein RBD , hFc Tag

SKU:  PME100658     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100656 cd164 sp1

ECD Proteins

Human CD164 Protein, hFc Tag

SKU:  PME100656     Target:  CD164     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100654 nefl sp1

ECD Proteins

Human NEFL (9-88) Protein, hFc Tag

SKU:  PME100654     Target:  NEFL     Tag:  C-Human Fc Tag

Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00

sp-pme100653 grp sp1

ECD Proteins

Human GRP Protein, hFc Tag

SKU:  PME100653     Target:  GRP     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100612 eva1c sp1

ECD Proteins

Human EVA1C Protein, hFc Tag

SKU:  PME100612     Target:  EVA1C     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100650 fzd10 sp1

ECD Proteins

Human FZD10 Protein, hFc Tag

SKU:  PME100650     Target:  FZD10     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100651 il22 sp1

ECD Proteins

Human IL22 Protein, hFc Tag

SKU:  PME100651     Target:  IL22     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

elisa-pme100652 tnfrsf25 elisa1

ECD Proteins

Human TNFRSF25 Protein, hFc Tag

SKU:  PME100652     Target:  TNFRSF25     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100645 siglec7 sp1

ECD Proteins

Human SIGLEC7 Protein, His Tag

SKU:  PME100645     Target:  SIGLEC7     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100644 cd23 sp1

ECD Proteins

Human CD23 Protein, His Tag

SKU:  PME100644     Target:  CD23     Tag:  N-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100643 cd205 sp1

ECD Proteins

Human CD205 Protein, His Tag

SKU:  PME100643     Target:  CD205     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100322 kras sp1

ECD Proteins

Human KRAS Protein, mFc Tag

SKU:  PME100322     Target:  KRAS     Tag:  C-mouse Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100901 adam9 sp1

ECD Proteins

Human ADAM9 Protein, His Tag

SKU:  PME100901     Target:  ADAM9     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100642 cd74 sp1

ECD Proteins

Human CD74 (73-296) Protein, hFc Tag

SKU:  PME100642     Target:  CD74     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100641 cd74 sp1

ECD Proteins

Human CD74 (73-232) Protein, hFc Tag

SKU:  PME100641     Target:  CD74     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100640 bst1 sp1

ECD Proteins

Human BST1 Protein, hFc Tag

SKU:  PME100640     Target:  BST1     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100639 il11 sp1

ECD Proteins

Human IL11 Protein, hFc Tag

SKU:  PME100639     Target:  IL11     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100638 ghr sp1

ECD Proteins

Human GHR Protein, hFc Tag

SKU:  PME100638     Target:  GHR     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100637 tslp sp1

ECD Proteins

Human TSLP Protein, hFc Tag

SKU:  PME100637     Target:  TSLP     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100636 gp6 sp1

ECD Proteins

Human GP6 Protein, hFc Tag

SKU:  PME100636     Target:  GP6     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100635 apcdd1 sp1

ECD Proteins

Human APCDD1 Protein, His Tag

SKU:  PME100635     Target:  APCDD1     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100633 trem2 sp1

ECD Proteins

Human TREM2 Protein, hFc Tag

SKU:  PME100633     Target:  TREM2     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme100632 gp1bb sp1

ECD Proteins

Human GP1BB Protein, hFc Tag

SKU:  PME100632     Target:  GP1BB     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100631 ephb2 sp1

ECD Proteins

Human EPHB2 Protein, His Tag

SKU:  PME100631     Target:  EPHB2     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00