ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-pme100709 ddr1 sp1

ECD Proteins

Human DDR1 Protein, His Tag

SKU:  PME100709     Target:  DDR1     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100704 gpr75 sp1

ECD Proteins

Human GPR75 Protein, hFc Tag

SKU:  PME100704     Target:  GPR75     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100703 b4galt1 sp1

ECD Proteins

Human B4GALT1 Protein, hFc Tag

SKU:  PME100703     Target:  B4GALT1     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100699 mmp13 sp1

ECD Proteins

Human MMP13 Protein, hFc Tag

SKU:  PME100699     Target:  MMP13     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100698 mmp13 sp1

ECD Proteins

Human MMP13 Protein, His Tag

SKU:  PME100698     Target:  MMP13     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100696 igfbp7 sp1

ECD Proteins

Human IGFBP7 Protein, hFc Tag

SKU:  PME100696     Target:  IGFBP7     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme102006 mbp sp1

ECD Proteins

Human MBP Protein, hFc Tag

SKU:  PME102006     Target:  MBP     Tag:  N-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme c100004 ncl sp1

ECD Proteins

Rhesus macaque NCL Protein, His Tag

SKU:  PME-C100004     Target:  NCL     Tag:  N-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-pme m100027 mfge8 sp1

ECD Proteins

Mouse MFGE8 Protein, His Tag

SKU:  PME-M100027     Target:  MFGE8     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100814 igf 1r sp1

ECD Proteins

Human IGF-1R Protein, His Tag

SKU:  PME100814     Target:  IGF-1R     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100693 cd33 sp1

ECD Proteins

Human CD33(140-259) Protein, hFc Tag

SKU:  PME100693     Target:  CD33     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100692 cdh1 sp1

ECD Proteins

Human CDH1 Protein, mFc Tag

SKU:  PME100692     Target:  CDH1     Tag:  C-Mouse Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100694 pglyrp1 sp1

ECD Proteins

Human PGLYRP1 Protein, hFc Tag

SKU:  PME100694     Target:  PGLYRP1     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme m100026 vwf sp1

ECD Proteins

Mouse VWF (23-2813) Protein, His Tag

SKU:  PME-M100026     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme m100025 vwf sp1

ECD Proteins

Mouse VWF (764-2813) Protein, His Tag

SKU:  PME-M100025     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme m100024 vwf sp1

ECD Proteins

Mouse VWF (23-763) Protein, His Tag

SKU:  PME-M100024     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100690 vwf sp1

ECD Proteins

Human VWF (23-2813) Protein, His Tag

SKU:  PME100690     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme100689 cd93 sp1

ECD Proteins

Human CD93 Protein, hFc Tag

SKU:  PME100689     Target:  CD93     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme100688 nucleocapsid sp1

ECD Proteins

SARS-CoV-2 (Omicron) Nucleocapsid, C-His Tag

SKU:  PME100688     Target:  Nucleocapsid     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme d100001 il18bp sp1

ECD Proteins

Canis lupus familiaris IL18BP Protein, hFc Tag

SKU:  PME-D100001     Target:  IL18BP     Tag:  C-Human Fc Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme c100003 il9 sp1

ECD Proteins

Cynomolgus IL9 Protein, hFc Tag

SKU:  PME-C100003     Target:  IL9     Tag:  C-Human Fc Tag

Price: 10μg ￥799.00; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme c100002 cxcl10 sp1

ECD Proteins

Cynomolgus CXCL10 Protein, hFc Tag

SKU:  PME-C100002     Target:  CXCL10     Tag:  C-Human Fc Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme c100001 il18bp sp1

ECD Proteins

Cynomolgus IL18BP Protein, hFc Tag

SKU:  PME-C100001     Target:  IL18BP     Tag:  C-Human Fc Tag

Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00

sp-pme m100023 rnase4 sp1

ECD Proteins

Mouse RNASE4 Protein, hFc Tag

SKU:  PME-M100023     Target:  RNASE4     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme m100022 lama4 sp1

ECD Proteins

Mouse LAMA4 Protein, His Tag

SKU:  PME-M100022     Target:  LAMA4     Tag:  C-6×His Tag

Price: 10μg ￥650.00 ; 50μg ￥2600.00 ; 100 μg ￥3800.00

sp-pme m100021 tenm4 sp1

ECD Proteins

Mouse TENM4 Protein, His Tag

SKU:  PME-M100021     Target:  TENM4     Tag:  N-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme m100020 hbegf sp1

ECD Proteins

Mouse HBEGF (24-160) Protein, His Tag

SKU:  PME-M100020     Target:  HBEGF     Tag:  C-6×His Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme m100017 ifnb1 sp1

ECD Proteins

Mouse IFNB1 Protein, His Tag

SKU:  PME-M100017     Target:  IFNB1     Tag:  C-6×His Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme m100016 gitr sp1

ECD Proteins

Mouse GITR Protein, hFc Tag

SKU:  PME-M100016     Target:  GITR     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme m100015 cd33 sp1

ECD Proteins

Mouse CD33 Protein, hFc Tag

SKU:  PME-M100015     Target:  CD33     Tag:  C-Human Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

sp-pme m100014 cd138 sp1

ECD Proteins

Mouse CD138 Protein, hFc Tag

SKU:  PME-M100014     Target:  CD138     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00

sp-pme100686 hbegf sp1

ECD Proteins

Human HBEGF Protein, hFc Tag

SKU:  PME100686     Target:  HBEGF     Tag:  C-Human Fc Tag

Price: 10μg ￥668.00 ; 50μg ￥2499.00 ; 100μg ￥3750.00