ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-pme m100049 cd48 sp1

ECD Proteins

Mouse CD48 Protein, hFc Tag

SKU:  PME-M100049     Target:  CD48     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100048 tim3 sp1

ECD Proteins

Mouse TIM3 Protein, hFc Tag

SKU:  PME-M100048     Target:  TIM3     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100047 b7 h2 sp1

ECD Proteins

Mouse B7-H2 Protein, hFc Tag

SKU:  PME-M100047     Target:  B7-H2     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100046 b7 2 sp1

ECD Proteins

Mouse B7-2 Protein, hFc Tag

SKU:  PME-M100046     Target:  B7-2     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100045 psca sp1

ECD Proteins

Mouse PSCA Protein, hFc Tag

SKU:  PME-M100045     Target:  PSCA     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100044 b7 1 sp1

ECD Proteins

Mouse B7-1 Protein, hFc Tag

SKU:  PME-M100044     Target:  B7-1     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100043 ox40 sp1

ECD Proteins

Mouse OX40 Protein, hFc Tag

SKU:  PME-M100043     Target:  OX40     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100042 cd40 sp1

ECD Proteins

Mouse CD40 Protein, hFc Tag

SKU:  PME-M100042     Target:  CD40     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100041 dnam 1 sp1

ECD Proteins

Mouse DNAM-1 Protein, hFc Tag

SKU:  PME-M100041     Target:  DNAM1     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100040 mesothelin sp1

ECD Proteins

Mouse Mesothelin Protein, His Tag

SKU:  PME-M100040     Target:  Mesothelin     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100039 qsox1 sp1

ECD Proteins

Mouse QSOX1 Protein, His Tag

SKU:  PME-M100039     Target:  QSOX1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100038 adam28 sp1

ECD Proteins

Mouse ADAM28 Protein, hFc Tag

SKU:  PME-M100038     Target:  ADAM28     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100773 ifnar1 sp1

ECD Proteins

Human IFNAR1 Protein, hFc Tag

SKU:  PME100773     Target:  IFNAR1     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100771 adamts1 sp1

ECD Proteins

Human ADAMTS1 Protein, His Tag

SKU:  PME100771     Target:  ADAMTS1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100770 tpsab1 sp1

ECD Proteins

Human TPSAB1 Protein, His Tag

SKU:  PME100770     Target:  TPSAB1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100769 fgf21 sp1

ECD Proteins

Human FGF21 Protein, hFc Tag

SKU:  PME100769     Target:  FGF21     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100768 b7 h7 sp1

ECD Proteins

Human B7-H7 Protein, hFc Tag

SKU:  PME100768     Target:  B7-H7     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100767 cd72 sp1

ECD Proteins

Human CD72 Protein, hFc Tag

SKU:  PME100767     Target:  CD72     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100766 trop2 sp1

ECD Proteins

Human Trop2 Protein, His Tag

SKU:  PME100766     Target:  Trop2     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100765 timp4 sp1

ECD Proteins

Human TIMP4 Protein, hFc Tag

SKU:  PME100765     Target:  TIMP4     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100764 itgax itgb2 sp1

ECD Proteins

Human ITGAX and ITGB2 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME100764     Target:  ITGAX and ITGB2     Tag:  C-6×His Tag and C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme100763 cldn5 sp1

ECD Proteins

Human CLDN5 Protein, hFc Tag

SKU:  PME100763     Target:  CLDN5     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100762 rnf43 sp1

ECD Proteins

Human RNF43 Protein, mFc Tag

SKU:  PME100762     Target:  RNF43     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100761 rnf43 sp1

ECD Proteins

Human RNF43 Protein, hFc Tag

SKU:  PME100761     Target:  RNF43     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100760 anxa1 sp1

ECD Proteins

Human ANXA1 Protein, His Tag

SKU:  PME100760     Target:  ANXA1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100759 liv 1 sp1

ECD Proteins

Human LIV-1 Protein, His Tag

SKU:  PME100759     Target:  LIV-1     Tag:  C-6×His Tag

Price: 10μg ￥854.00 ; 50μg ￥3133.00 ; 100 μg ￥4699.00

sp-pme100753 adam15 sp1

ECD Proteins

Human ADAM15 Protein, hFc Tag

SKU:  PME100753     Target:  ADAM15     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100661 eda sp1

ECD Proteins

Human EDA Protein, hFc Tag

SKU:  PME100661     Target:  EDA     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100037 ncl sp1

ECD Proteins

Mouse NCL Protein, His Tag

SKU:  PME-M100037     Target:  NCL     Tag:  N-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100063 cldn6 sp1

ECD Proteins

Human CLDN6 Protein, mFc Tag

SKU:  PME100063     Target:  CLDN6     Tag:  C-Mouse Fc Tag

Price: 10μg ￥673.00 ; 50μg ￥2466.00 ; 100 μg ￥3699.00

fc-PME100559P PE GPRC5D mFc FLOW Fig.1

ECD Proteins

PE-conjugated Human GPRC5D Protein, mFc Tag

SKU:  PME100559P     Target:  GPRC5D     Tag:  C-Mouse Fc Tag

Price: 25test ￥3070.00

sp-pme100772 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Omicron BA.2) S protein RBD, hFc Tag

SKU:  PME100772     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00