ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

人、鼠、猴、犬源重组蛋白：抗体药物研发的隐藏关键

在抗体药物（therapeutic antibody）研发流程中，…

14
11 月
靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

04
12 月

产品列表

sp-PME30055 CC87 P05000 SDS PAGE 0

ECD Proteins

Human IFNW1 (C-6His) Protein

SKU:  PME30055     Target:  IFNW1     Tag:  C-6×His Tag

Price: 10 μg ￥760.00 ; 50 μg ￥2280.00

sp-PME30049 CB04 Q9UHF4 SDS PAGE 0

ECD Proteins

Human IL-20RA (C-Fc) Protein

SKU:  PME30049     Target:  IL-20RA     Tag:  C-Fc Tag

Price: 10 μg ￥760.00 ; 50 μg ￥2000.00

sp-PME30034 CC42 Q08334 SDS PAGE 0

ECD Proteins

Human IL-10RB (C-Fc) Protein

SKU:  PME30034     Target:  IL-10RB     Tag:  C-Fc Tag

Price: 10 μg ￥400.00 ; 50 μg ￥1200.00

sp-PME101158 NLRP3 Fig.1 SDS PAGE 1

ECD Proteins

Human NLRP3 Protein, Flag Tag

SKU:  PME101158     Target:  NLRP3     Tag:  C-Flag Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100076 asgr1 sp1

ECD Proteins

Mouse ASGR1 Protein, His Tag

SKU:  PME-M100076     Target:  ASGR1     Tag:  N-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100075 bmp6 sp1

ECD Proteins

Mouse BMP6 Protein, His Tag

SKU:  PME-M100075     Target:  BMP6     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme c100011 btn3a1 sp1

ECD Proteins

Cynomolgus BTN3A1 Protein, His Tag

SKU:  PME-C100011     Target:  BTN3A1     Tag:  C-6×His Tag

Price: 10μg ￥799.00; 50μg ￥2666.00 ; 100 μg ￥3999.00

sp-pme101150 ceacam8 sp1

ECD Proteins

Human CEACAM8 Protein, His Tag

SKU:  PME101150     Target:  CEACAM8     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101149 fgfr3 sp1

ECD Proteins

Human FGFR3 Protein, His Tag

SKU:  PME101149     Target:  FGFR3     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101148 pcdh18 sp1

ECD Proteins

Human PCDH18 Protein, His Tag

SKU:  PME101148     Target:  PCDH18     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101147 dlk1 sp1

ECD Proteins

Human DLK1 Protein, His Tag

SKU:  PME101147     Target:  DLK1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101145 bcam sp1

ECD Proteins

Human BCAM Protein, His Tag

SKU:  PME101145     Target:  BCAM     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101144 cd106 sp1

ECD Proteins

Human CD106 Protein, hFc Tag

SKU:  PME101144     Target:  CD106     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101143 lamp3 sp1

ECD Proteins

Human LAMP3 Protein, His Tag

SKU:  PME101143     Target:  LAMP3     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101142 btn3a2 sp1

ECD Proteins

Human BTN3A2 Protein, His Tag

SKU:  PME101142     Target:  BTN3A2     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101141 asgr1 sp1

ECD Proteins

Human ASGR1 Protein, His Tag

SKU:  PME101141     Target:  ASGR1     Tag:  N-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101140 furin sp1

ECD Proteins

Human FURIN Protein, His Tag

SKU:  PME101140     Target:  FURIN     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101125 igfbp2 sp1

ECD Proteins

Human IGFBP2 Protein, His Tag

SKU:  PME101125     Target:  IGFBP2     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101139 btn3a3 sp1

ECD Proteins

Human BTN3A3 Protein, His Tag

SKU:  PME101139     Target:  BTN3A3     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101138 cd94 sp1

ECD Proteins

Human CD94 Protein, hFc Tag

SKU:  PME101138     Target:  CD94     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101137 tspan8 sp1

ECD Proteins

Human TSPAN8 Protein, hFc Tag

SKU:  PME101137     Target:  TSPAN8     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101136 pyy sp1

ECD Proteins

Human PYY Protein, hFc Tag

SKU:  PME101136     Target:  PYY     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101135 retn sp1

ECD Proteins

Human RETN Protein, hFc Tag

SKU:  PME101135     Target:  RETN     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101134 klk3 sp1

ECD Proteins

Human KLK3 Protein, His Tag

SKU:  PME101134     Target:  KLK3     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101133 il12b sp1

ECD Proteins

Human IL12B Protein, His Tag

SKU:  PME101133     Target:  IL12B     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101111 cdh6 sp1

ECD Proteins

Human CDH6 Protein, His Tag

SKU:  PME101111     Target:  CDH6     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101132 areg sp1

ECD Proteins

Human AREG Protein, hFc Tag

SKU:  PME101132     Target:  AREG     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101131 sema4d sp1

ECD Proteins

Human SEMA4D Protein, His Tag

SKU:  PME101131     Target:  SEMA4D     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101129 cd59 sp1

ECD Proteins

Human CD59 Protein, hFc Tag

SKU:  PME101129     Target:  CD59     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101128 il13 sp1

ECD Proteins

Human IL13(35-146) Protein, hFc Tag

SKU:  PME101128     Target:  IL13     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101127 ppt1 sp1

ECD Proteins

Human PPT1 Protein, His Tag

SKU:  PME101127     Target:  PPT1     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101126 il18bp sp1

ECD Proteins

Human IL18BP Protein, hFc Tag

SKU:  PME101126     Target:  IL18BP     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00