ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

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在抗体药物（therapeutic antibody）研发流程中，…

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靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-pme101124 npr1 sp1

ECD Proteins

Human NPR1 Protein, hFc Tag

SKU:  PME101124     Target:  NPR1     Tag:  C-Human Fc Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101123 bmp6 sp1

ECD Proteins

Human BMP6 Protein, His Tag

SKU:  PME101123     Target:  BMP6     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101120 bst2 sp1

ECD Proteins

Human BST2 Protein, hFc Tag

SKU:  PME101120     Target:  BST2     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101119 fam3d sp1

ECD Proteins

Human FAM3D Protein, hFc Tag

SKU:  PME101119     Target:  FAM3D     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101118 f2rl1 sp1

ECD Proteins

Human F2RL1 Protein, hFc Tag

SKU:  PME101118     Target:  F2RL1     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101117 amigo2 sp1

ECD Proteins

Human AMIGO2 Protein, His Tag

SKU:  PME101117     Target:  AMIGO2     Tag:  C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00

sp-pme101116 scg3 sp1

ECD Proteins

Human SCG3 Protein, His Tag

SKU:  PME101116     Target:  SCG3     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101115 osmr sp1

ECD Proteins

Human OSMR Protein, His Tag

SKU:  PME101115     Target:  OSMR     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101114 hsd17b13 sp1

ECD Proteins

Human HSD17B13 Protein, Flag Tag

SKU:  PME101114     Target:  HSD17B13     Tag:  C-3×Flag Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101112 ccr4 sp1

ECD Proteins

Human CCR4(1-39) Protein, mFc Tag

SKU:  PME101112     Target:  CCR4     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101110 gpr87 sp1

ECD Proteins

Human GPR87 Protein, mFc Tag

SKU:  PME101110     Target:  GPR87     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101109 ccr2 sp1

ECD Proteins

Human CCR2 Protein, mFc Tag

SKU:  PME101109     Target:  CCR2     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101108 gprc5c sp1

ECD Proteins

Human GPRC5C Protein, mFc Tag

SKU:  PME101108     Target:  GPRC5C     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101107 gprc5b sp1

ECD Proteins

Human GPRC5B Protein, mFc Tag

SKU:  PME101107     Target:  GPRC5B     Tag:  C-Mouse Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme101106 gprc5a sp1

ECD Proteins

Human GPRC5A Protein, mFc Tag

SKU:  PME101106     Target:  GPRC5A     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme101105 ccr6 sp1

ECD Proteins

Human CCR6 Protein, mFc Tag

SKU:  PME101105     Target:  CCR6     Tag:  C-Mouse Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme100799 cxcr3 sp1

ECD Proteins

Human CXCR3 Protein, mFc Tag

SKU:  PME100799     Target:  CXCR3     Tag:  C-Mouse Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2666.00 ; 100μg ￥3999.00

sp-pme101146 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Omicron BA.5) S protein RBD, hFc Tag

SKU:  PME101146     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100072 baff sp1

ECD Proteins

Mouse BAFF Protein, hFc Tag

SKU:  PME-M100072     Target:  BAFF     Tag:  N-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme m100071 4 1bb ligand sp1

ECD Proteins

Mouse 4-1BB Ligand Protein, hFc Tag

SKU:  PME-M100071     Target:  4-1BB Ligand     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100070 nkg2d sp1

ECD Proteins

Mouse NKG2D Protein, hFc Tag

SKU:  PME-M100070     Target:  NKG2D     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100069 ror1 sp1

ECD Proteins

Mouse ROR1 Protein, His Tag

SKU:  PME-M100069     Target:  ROR1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100068 cd40 ligand sp1

ECD Proteins

Mouse CD40 Ligand Protein, hFc Tag

SKU:  PME-M100068     Target:  CD40 Ligand     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100067 ox40 ligand sp1

ECD Proteins

Mouse OX40 Ligand Protein, hFc Tag

SKU:  PME-M100067     Target:  OX40 Ligand     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100066 gitr ligand sp1

ECD Proteins

Mouse GITR Ligand Protein, hFc Tag

SKU:  PME-M100066     Target:  GITR Ligand     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100065 taci sp1

ECD Proteins

Mouse TACI Protein, hFc Tag

SKU:  PME-M100065     Target:  TACI     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100064 cd34 sp1

ECD Proteins

Mouse CD34 Protein, hFc Tag

SKU:  PME-M100064     Target:  CD34     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100063 il2ra sp1

ECD Proteins

Mouse IL2RA Protein, hFc Tag

SKU:  PME-M100063     Target:  IL2RA     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100062 egfr sp1

ECD Proteins

Mouse EGFR Protein, His Tag

SKU:  PME-M100062     Target:  EGFR     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100061 cd22 sp1

ECD Proteins

Mouse CD22 Protein, His Tag

SKU:  PME-M100061     Target:  CD22     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

sp-pme m100060 ceacam5 sp1

ECD Proteins

Mouse CEACAM5 Protein, His Tag

SKU:  PME-M100060     Target:  CEACAM5     Tag:  C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

sp-pme100798 slpi sp1

ECD Proteins

Human SLPI Protein, hFc Tag

SKU:  PME100798     Target:  SLPI     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00