ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

优势免疫原应用数据蛋白活性数据热门靶点产品列表

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

蛋白活性数据

Human 4-1BB(46-86) Protein (PME101695)

Mouse 4-1BB Protein (PME-M100007)

PME-C100023-4-1BB-Fig.1-SDS-PAGE-1-768x768

Cynomolgus 4-1BB Protein (PME-C100023)

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热点速递

人、鼠、猴、犬源重组蛋白：抗体药物研发的隐藏关键

在抗体药物（therapeutic antibody）研发流程中，…

14
11 月

靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

04
12 月

产品列表

ECD Proteins

Human VPS4 Protein, His Tag

SKU: PME101167 Target: VPS4 Tag: C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

ECD Proteins

Human LGALS3BP Protein, Flag Tag

SKU: PME101187 Target: LGALS3BP Tag: C-3×Flag Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

ECD Proteins

Human IL8 Protein, His Tag

SKU: PME101168 Target: IL8 Tag: C-6×His Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

ECD Proteins

Human PDGFD(250-370) Protein, hFc Tag

SKU: PME101172 Target: PDGFD Tag: C-Human Fc Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

ECD Proteins

Human PDGFD(19-370) Protein, His Tag

SKU: PME101166 Target: PDGFD Tag: C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

ECD Proteins

Human IL17F Protein, His Tag

SKU: PME101165 Target: IL17F Tag: N-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

ECD Proteins

Human NRP2 Protein, His Tag

SKU: PME101164 Target: NRP2 Tag: C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

ECD Proteins

Human LRG1 Protein, His Tag

SKU: PME101163 Target: LRG1 Tag: C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

ECD Proteins

Human AGTR1 Protein, hFc Tag

SKU: PME101162 Target: AGTR1 Tag: C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

ECD Proteins

Human IL10 Protein, His Tag

SKU: PME101160 Target: IL10 Tag: N-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

ECD Proteins

Human FGL1 Protein, His Tag

SKU: PME101159 Target: FGL1 Tag: C-6×His Tag

Price: 10μg ￥727.00 ; 50μg ￥2700.00 ; 100 μg ￥3999.00

ECD Proteins

Human PTTG1IP Protein, hFc Tag

SKU: PME101157 Target: PTTG1IP Tag: C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

ECD Proteins

Human IL9 Protein, hFc Tag

SKU: PME101156 Target: IL9 Tag: C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

ECD Proteins

Human IL9 Protein, His Tag

SKU: PME101155 Target: IL9 Tag: C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

ECD Proteins

Human ULBP2 Protein, His Tag

SKU: PME101154 Target: ULBP2 Tag: C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100 μg ￥3499.00

ECD Proteins

Human PRTN3 Protein, His Tag

SKU: PME101153 Target: PRTN3 Tag: C-6×His Tag

Price: 10μg ￥782.00 ; 50μg ￥2866.00 ; 100 μg ￥4299.00