ECD 蛋白（Extracellular Domain Proteins）

什么是ECD蛋白？

ECD（Extracellular Domain）蛋白是指膜蛋白位于细胞外侧与外界环境直接接触的结构区域，通常承担配体结合、信号识别、配体结合及细胞间通讯等关键功能。许多重要的受体类药物靶点，如G蛋白偶联受体（GPCR）、受体酪氨酸激酶（RTK）及免疫检查点分子（如PD-1、CTLA-4等），其生物活性主要由胞外域介导。ECD通过特异性识别配体或抗体，不仅参与生理信号传导，还在免疫应答、肿瘤发生及病毒感染等过程中发挥重要作用。

ECD蛋白是膜蛋白抗体开发中最常用的免疫原与筛选抗原之一。相较于全长膜蛋白，ECD 蛋白在表达、纯化和检测体系兼容性方面更具优势，广泛应用于抗体早期筛选、亲和力评价及功能验证。

the figure of extra-cellular domain structure

特色与应用

特色

缔码生物的ECD蛋白主要是针对于单次跨膜蛋白，利用其哺乳动物表达系统已成功表达出1600+高纯度ECD蛋白，覆盖900+靶点。其中，有500+ECD蛋白已成功开发出对应流式抗体。缔码生物所有ECD蛋白产品均经SDS-PAGE纯度验证及ELISA活性，可支持抗体筛选、功能验证及结构研究等多种应用。

哺乳动物细胞表达

天然构象及完整翻译后修饰

成功验证可做免疫原用于治疗性抗体开发

覆盖1000+药物靶点

针对同一靶点提供不同种属及不同片段蛋白

可用于种属交叉验证及抗体表位筛选

支持多种治疗性抗体功能筛选验证

SDS-PAGE | ELISA | Flow Cyt | SPR

免疫原应用数据

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

the validation data of FACS antibody were generated using LAG3 protein

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

蛋白活性数据

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

点击查看ECD蛋白活性及稳定性验证数据

热门靶点分类

免疫检查点

免疫检查点是一类调节肿瘤免疫微环境的关键蛋白，包括可增强免疫应答的共刺激型检查点（如 OX40）和可抑制免疫反应的共抑制型检查点（如 PD-1）。

点击查看免疫检查点靶点

血液肿瘤靶点

血液肿瘤靶点（如：CD19、CD20和CD38）是与白血病、淋巴瘤和多发性骨髓瘤等血液癌症相关的特定分子，用于靶向治疗，如单克隆抗体。

点击查看血液肿瘤靶点

实体瘤靶点

实体肿瘤靶点（如HER2、EGFR和VEGF）是存在于实体肿瘤细胞表面的特定分子，常见于乳腺癌、肺癌和结直肠癌等，常通过单抗和小分子药进行靶向治疗。

点击查看实体瘤靶点

热点速递

人、鼠、猴、犬源重组蛋白：抗体药物研发的隐藏关键

在抗体药物（therapeutic antibody）研发流程中，…

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11 月
靶点蛋白不同结构域蛋白片段在治疗性抗体开发中的应用

在抗体药物开发过程中，筛选出具有特殊功能和表位的抗体往往面临一些挑…

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产品列表

sp-PME C100012 ADAM9 Fig.1 SDS PAGE 1

ECD Proteins

Cynomolgus ADAM9 Protein, His Tag

SKU:  PME-C100012     Target:  ADAM9     Tag:  C-6×His Tag

Price: 10μg ￥668.00; 50μg ￥2310.00 ; 100 μg ￥3499.00

sp-PME101302 GPR81 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR81 Protein, hFc Tag

SKU:  PME101302     Target:  GPR81     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101301 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(110-139) Protein, hFc Tag

SKU:  PME101301     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101300 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(77-106) Protein, hFc Tag

SKU:  PME101300     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101299 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(44-72) Protein, hFc Tag

SKU:  PME101299     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101298 p16 Fig.1 SDS PAGE 1

ECD Proteins

Human p16(11-40) Protein, hFc Tag

SKU:  PME101298     Target:  p16     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101297 IBSP Fig.1 SDS PAGE 1

ECD Proteins

Human IBSP Protein, His Tag

SKU:  PME101297     Target:  IBSP     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101296 IL4 Fig.1 SDS PAGE 1

ECD Proteins

Human IL4 Protein, His Tag

SKU:  PME101296     Target:  IL4     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101295 ARTN Fig.1 SDS PAGE 1

ECD Proteins

Human ARTN Protein, hFc Tag

SKU:  PME101295     Target:  ARTN     Tag:  C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101294 S100A9 Fig.1 SDS PAGE 1

ECD Proteins

Human S100A9 Protein, hFc Tag

SKU:  PME101294     Target:  S100A9     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101291 FSTL1 Fig.1 SDS PAGE 1

ECD Proteins

Human FSTL1 Protein, His Tag

SKU:  PME101291     Target:  FSTL1     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101289 CALCA Fig.1 SDS PAGE 1

ECD Proteins

Human CALCA(26-141) Protein, hFc Tag

SKU:  PME101289     Target:  CALCA     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101288 CXCL5 Fig.1 SDS PAGE 1

ECD Proteins

Human CXCL5 Protein, hFc Tag

SKU:  PME101288     Target:  CXCL5     Tag:  N-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101287 DSG2 Fig.1 SDS PAGE 1

ECD Proteins

Human DSG2(24-609) Protein, His Tag

SKU:  PME101287     Target:  DSG2     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101286 DSG2 Fig.1 SDS PAGE 1

ECD Proteins

Human DSG2(50-609) Protein, His Tag

SKU:  PME101286     Target:  DSG2     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101285 FGF19 Fig.1 SDS PAGE 1

ECD Proteins

Human FGF19 Protein, His Tag

SKU:  PME101285     Target:  FGF19     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101284 PTGFRN Fig.1 SDS PAGE 1

ECD Proteins

Human PTGFRN Protein, His Tag

SKU:  PME101284     Target:  PTGFRN     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101283 CD300LF Fig.1 SDS PAGE 1

ECD Proteins

Human CD300LF Protein, hFc Tag

SKU:  PME101283     Target:  CD300LF     Tag:  C-Human Fc Tag

Price: 10μg ￥690.00 ; 50μg ￥2500.00 ; 100μg ￥3699.00

sp-PME101282 CHRM2 Fig.1 SDS PAGE 1

ECD Proteins

Human CHRM2 Protein, hFc Tag

SKU:  PME101282     Target:  CHRM2     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101281 PF4V1 Fig.1 SDS PAGE 1

ECD Proteins

Human PF4V1 Protein, hFc Tag

SKU:  PME101281     Target:  PF4V1     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-pme100730 cd3d cd3e sp1

ECD Proteins

Human CD3D Protein, His Tag and Human CD3E Protein, hFc Tag

SKU:  PME100730     Target:  CD3D; CD3E     Tag:  C-6×His Tag and C-Human Fc Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101269 GLP1R Fig.1 SDS PAGE 1

ECD Proteins

Human GLP1R Protein, hFc Tag

SKU:  PME101269     Target:  GLP1R     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101264 LRP10 Fig.1 SDS PAGE 1

ECD Proteins

Human LRP10 Protein, His Tag

SKU:  PME101264     Target:  LRP10     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101305 CGRP Fig.1 SDS PAGE 1

ECD Proteins

Human CGRP Protein, hFc Tag

SKU:  PME101305     Target:  CGRP     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101293 CDH19 Fig.1 SDS PAGE 1

ECD Proteins

Human CDH19 Protein, His Tag

SKU:  PME101293     Target:  CDH19     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101290 FCGR3B Fig.1 SDS PAGE 1

ECD Proteins

Human FCGR3B Protein, His Tag

SKU:  PME101290     Target:  FCGR3B     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00

sp-PME101280 ECSCR Fig.1 SDS PAGE 1

ECD Proteins

Human ECSCR Protein, hFc Tag

SKU:  PME101280     Target:  ECSCR     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101279 TAFA5 Fig.1 SDS PAGE 1

ECD Proteins

Human TAFA5 Protein, hFc Tag

SKU:  PME101279     Target:  TAFA5     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101278 FCGR3B Fig.1 SDS PAGE 1

ECD Proteins

Human FCGR3B Protein, hFc Tag

SKU:  PME101278     Target:  FCGR3B     Tag:  C-Human Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101277 GPR75 Fig.1 SDS PAGE 1

ECD Proteins

Human GPR75 Protein, mFc Tag

SKU:  PME101277     Target:  GPR75     Tag:  C-Mouse Fc Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101276 CPM Fig.1 SDS PAGE 1

ECD Proteins

Human CPM Protein, His Tag

SKU:  PME101276     Target:  CPM     Tag:  C-6×His Tag

Price: 10μg ￥600.00 ; 50μg ￥2199.00 ; 100μg ￥3299.00

sp-PME101275 MANSC1 Fig.1 SDS PAGE 1

ECD Proteins

Human MANSC1 Protein, His Tag

SKU:  PME101275     Target:  MANSC1     Tag:  C-6×His Tag

Price: 10μg ￥636.00 ; 50μg ￥2333.00 ; 100μg ￥3499.00