| Tag | C-Flag and Avi Tag |
|---|---|
| Target | |
| Synonyms | OAZON; claudin-2 |
| Description | Biotinylated Human CLDN2 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | Q8NHS1 |
| Expression Host | HEK293 |
| Protein Families | Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction |
| Molecular Weight | The human full length CLDN2 Protein has a MW of 29.3 kDa. |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010] |
| Usage | Research use only |
| Conjugate | Biotinylated |
Biotinylated Human CLDN2 full length protein-synthetic nanodisc
10 μg ￥6930.00 ; 50 μg ￥33000.00 ; 100 μg ￥60500.00
Product Data Dima FAQ
图片 Dima FAQ
Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Biotinylated Human CLDN2 full length protein-synthetic nanodisc (FLP100082B) can bind Anti-Flag Rabbit mAb in a linear range of 0.13-16 ng/mL.
Figure 2. ELISA plate pre-coated by 1 μg/mL (100 μL/well) Streptavidin can bind Biotinylated Human CLDN2 full length protein-synthetic nanodisc (FLP100082B) in a linear range of 16-400 ng/mL. In order to specifically detect FLP100082B, Anti-Flag Rabbit antibody was used as detection antibody.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Anti-flag Rabbit mAb can bind Biotinylated Human CLDN2 full length protein-synthetic nanodisc (FLP100082B) in a linear range of 16-400 ng/mL. In order to specifically detect FLP100082B, HRP Conjugated Streptavidin was used as detection antibody.
Figure 4. Biotinylated Human CLDN2-Nanodisc, Flag Tag on SDS-PAGE
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