Tag | C-Flag Taq |
---|---|
Target | |
Synonyms | OAZON |
Description | Human CLDN2 full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | P57739 |
Expression Host | HEK293 |
Protein Families | Transmembrane |
Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction |
Molecular Weight | The human full length CLDN2 protein has a MW of 24.5 kDa |
Background | This protein belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Usage | Research use only |
Human CLDN2 full length protein-synthetic nanodisc
Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00
Product Data
图片
Figure1. Elisa plates were pre-coated with Flag Tag CLDN2-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CLDN2-Nanodisc is 6.681ng/ml.
Figure2. Human CLDN2-Nanodisc, Flag Tag on SDS-PAGE
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