| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | PORIN; VDAC-1 |
| Description | Human VDAC1-Strep full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P21796 |
| Expression Host | HEK293 |
| Protein Families | Ion Channels: Other |
| Protein Pathways | Calcium signaling pathway, Huntington's disease, Parkinson's disease |
| Molecular Weight | The human full length VDAC1-Strep protein has a MW of 30.8 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human VDAC1-Strep full length protein-synthetic nanodisc
Price: 10 μg ￥5670.00 ;50 μg ￥27000.00 ; 100 μg ￥49500.00
Product Data Dima FAQ
图片 Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag VDAC1-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with VDAC1-Strep-nanodisc is 5.450ng/ml.
Figure 2. Human VDAC1-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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