| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | ANKTM1; FEPS; FEPS1 |
| Description | Human TRPA1-Strep full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | O75762 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Protein Pathways | N/A |
| Molecular Weight | The human full length TRPA1-Strep protein has a MW of 127.5 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The structure of the protein is highly related to both the protein ankyrin and transmembrane proteins. This protein is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human TRPA1-Strep full length protein-synthetic nanodisc
Price: 10 μg ￥5670.00 ;50 μg ￥27000.00 ; 100 μg ￥49500.00
Product Data Dima FAQ
图片 Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag TRPA1-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TRPA1-Strep-nanodisc is 6.181ng/ml.
Figure 2. Human TRPA1-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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