| Target | |
|---|---|
| Synonyms | IL-2 R beta & IL-2 R gamma,IL-2RB & IL-2RG |
| Description | Recombinant human IL2RB-knob protein with C-terminal 6×His tag and human IL2RG-Hole protein with C-terminal Strep tag |
| Delivery | In STOCK |
| Uniprot ID | P14784 and P31785 |
| Expression Host | HEK293 |
| Tag | C-6×His tag and C-Strep tag |
| Molecular Characterization | IL2RB(Ala27-Asp239) knob 6×His tag IL2RG(Leu23-Asn254) Hole Strep tag |
| Molecular Weight | The protein has a predicted molecular mass of 52.0 kDa and 56.7 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Background | The IL2RB and IL2RG heterodimer is composed of interleukin-2 receptor subunit beta (IL2RB/CD122) and the common gamma-chain receptor subunit (IL2RG/CD132). Together, these subunits form the intermediate-affinity interleukin-2 receptor and constitute the signaling components of the high-affinity IL-2 receptor complex when associated with IL2RA/CD25. IL2RB and IL2RG are also essential components of the interleukin-15 receptor complex. Upon ligand-induced receptor assembly, IL2RB and IL2RG activate JAK1 and JAK3, leading to downstream signaling through the STAT5, PI3K–AKT, and MAPK pathways. These signaling pathways regulate the proliferation, survival, differentiation, and effector functions of T cells, natural killer cells, and other immune cells. Dysregulation of IL-2 and IL-15 receptor signaling is associated with autoimmune diseases, immunodeficiency, inflammation, and cancer. Recombinant IL2RB and IL2RG heterodimer protein is suitable for cytokine–receptor interaction studies, binding and functional assays, antibody screening, drug discovery, and the development of IL-2- or IL-15-based therapeutics. |
| Usage | Research use only |
| Conjugate | Unconjugated |
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