Tag | C-Flag Taq |
---|---|
Target | |
Synonyms | EX33; GPCR4 |
Description | Human GPR84 full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | Q9NQS5 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | N/A |
Molecular Weight | The human full length GPR84 protein has a MW of 43.7 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | Receptor for medium-chain free fatty acid (FFA) with carbon chain lengths of C9 to C14. Capric acid (C10:0), undecanoic acid (C11:0) and lauric acid (C12:0) are the most potent agonists. Not activated by short-chain and long-chain saturated and unsaturated FFAs. Activation by medium-chain free fatty acid is coupled to a pertussis toxin sensitive G(i/o) protein pathway. May have important roles in processes from fatty acid metabolism to regulation of the immune system. |
Usage | Research use only |
Human GPR84 full length protein-synthetic nanodisc
Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00
Product Data
图片
Figure1. Elisa plates were pre-coated with Flag Tag GPR84-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPR84-Nanodisc is 2.893ng/ml.
Figure2. WB analysis of Human GPR84-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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