Tag | C-Flag Taq |
---|---|
Target | |
Synonyms | FzE3 |
Description | Human FZD7 full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | O75084 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway |
Molecular Weight | The human full length FZD7 protein has a MW of 63.6 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. |
Usage | Research use only |
Human FZD7 full length protein-synthetic nanodisc
Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00
Product Data
图片
Figure1. Elisa plates were pre-coated with Flag Tag FZD7-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with FZD7-Nanodisc is 1.783ng/ml.
Figure2. Human FZD7-Nanodisc, Flag Tag on SDS-PAGE
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