Recombinant human CTLA-4 protein with C-terminal mouse Fc and 6×His tag
C-Mouse Fc and 6×His Tag
CTLA-4(Gln36-Ser161) mFc(Pro99-Lys330) 6×His
The protein has a predicted molecular mass of 54 kDa after removal of the signal peptide.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Research use only
Human CTLA-4 Protein, mFc-His Tag
Price: 10μg ￥909.00 ; 50μg ￥3333.00 ; 100 μg ￥4999.00
Figure 1. Human CTLA-4 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-1, hFc tagged protein (PME100473) can bind Human CTLA4, mFc-His tagged protein (PME100017) in a linear range of 0.048-2.094 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human B7-2, hFc tagged protein (PME100474) can bind Human CTLA4, mFc-His tagged protein (PME100017) in a linear range of 0.048-2.694 ng/ml.
Figure 4. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA-4, mFc-His tagged protein (PME100017) can bind Anti-CTLA-4 Neutralizing antibody (BME100022) in a linear range of 0.13-16.0 ng/ml.
Figure 5. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, mFc-His tagged protein (PME100017) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
Figure 6. Flow cytometry analysis with 1ug/ml Human CTLA4 Protein, mFc-His tag (PME100017) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).