Clone ID | DM51 |
---|---|
Target | |
Synonyms | CTLA4; CD152 |
Host Species | Rabbit |
Description | Anti-CTLA-4 antibody(DM51); Rabbit mAb |
Delivery | In Stock |
Uniprot ID | P16410 |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Reactivity | Human |
Applications | ELISA; Flow Cyt |
Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain; a transmembrane domain; and a cytoplasmic tail. Alternate transcriptional splice variants; encoding different isoforms; have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond; while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus; Graves disease; Hashimoto thyroiditis; celiac disease; systemic lupus erythematosus; thyroid-associated orbitopathy; and other autoimmune diseases. |
Usage | Research use only |
Conjugate | Unconjugated |
Anti-CTLA-4 antibody(DM51); Rabbit mAb
Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
Product Data
图片
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA-4 protein, mFc-His tagged protein (PME100017) can bind Rabbit anti-CTLA-4 monoclonal antibody(clone: DM50) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CTLA-4 (B) were surface stained with Rabbit anti-CTLA-4 monoclonal antibody 1μg/ml (clone: DM50) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CTLA-4 monoclonal antibody ( clone: DM51 ) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti-CTLA-4 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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