Clone ID | DM81 |
---|---|
Target | |
Synonyms | HAVCR2; TIM3; TIMD3; FLJ14428; KIM3 |
Host Species | Rabbit |
Description | Anti-TIM3 antibody(DM81); Rabbit mAb |
Delivery | In Stock |
Uniprot ID | Q8TDQ0 |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Reactivity | Human |
Applications | ELISA; Flow Cyt |
Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein encoded by this gene belongs to the immunoglobulin superfamily; and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions; whereas; Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation; and inhibits Th1-mediated auto- and alloimmune responses; and promotes immunological tolerance. |
Usage | Research use only |
Anti-TIM3 antibody(DM81); Rabbit mAb
Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00
Product Data
图片
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human TIM3 protein, mFc-His tagged protein PME100030 can bind Rabbit anti-TIM3 monoclonal antibody ( clone: DM81) in a linear range of 0.32-40 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human TIM3 (B) were surface stained with Rabbit anti-TIM3 monoclonal antibody 1μg/ml ( clone: DM81) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry analysis of antigen binding of rabbit anti-human TIM3 mAb(DME100081).
(A) DME100081 does not bind to 293T cells that do not express TIM3.
(B) A clear peak shift of DME100081 was seen compared to the control when incubated with TIM3-expressing 8226 cells, indicating strong binding of DME100081 to TIM3. Antibodies were incubated at 10 μg/mL.
相关产品
Overexpression Stable Cell Line
SKU: CEL100031 Target: TIM3
Application: FACS Data
Price: Inquiry
Monoclonal Antibodies
SKU: DME100081B Target: TIM3
Application: ELISA; Flow Cyt
Price: 10μg ¥1030.00 ; 50μg ¥3380.00 ; 100 μg ¥6080.00 ; 500 μg ¥19880.00
ECD Proteins
SKU: PME101442 Target: TIM3
Price: 10μg ¥818.00 ; 50μg ¥2999.00 ; 100 μg ¥4499.00
Biosimilar reference antibodies
SKU: BME100681B Target: TIM3
Application: N/A
Price: 50 μg ¥890.00 ; 100 μg ¥1699.00
Biosimilar reference antibodies
SKU: BME100173 Target: TIM3
Application: ELISA, Flow Cyt
Price: 50 μg ¥800.00 ; 100 μg ¥1500.00