| Target | |
|---|---|
| Synonyms | HAVCR2;TIM3;TIMD3;FLJ14428;KIM3 |
| Description | Recombinant human TIM3 protein with C-terminal mouse Fc and 6×His tag |
| Delivery | In Stock |
| Uniprot ID | Q8TDQ0 |
| Expression Host | HEK293 |
| Tag | C-Mouse Fc and 6×His Tag |
| Molecular Characterization | TIM3(Ser22-Gly202) mFc(Pro99-Lys330) 6×His tag |
| Molecular Weight | The protein has a predicted molecular mass of 47.5 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. |
| Usage | Research use only |
Human TIM3 Protein, mFc-His Tag
Price: 10μg ￥818.00 ; 50μg ￥2999.00 ; 100 μg ￥4499.00
Product Data
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Figure 1. Human TIM3 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human TIM3, mFc-His tagged protein (PME100030) can bind Anti-TIM3 Neutralizing antibody (BME100008) in a linear range of 0.64-80 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TIM3 Protein, mFc-His Tag (PME100030) can bind Anti-TIM3(sabatolimab biosimilar) mAb (BME100173) in a linear range of 0.64–16 ng/mL.