兔单克隆抗体

与其他动物相比,兔拥有独特的B细胞发育过程,并且比啮齿动物与人类的亲缘性更远。除此之外,兔B细胞具有双重亲和力成熟机制,包括基因转换和SHM(体细胞超突变);同时,兔IgG的蛋白结构与鼠IgG不同,体现在其只拥有一个亚型IgG,兔轻链上特有的额外二硫键,以及CDR3的长度和序列差异很大等方面。这些不同之处使得兔单抗具有更高的亲和力和特异性,也更有可能筛选出具有差异化的抗体。缔码生物创新性的兔重组单克隆抗体平台,聚焦肿瘤免疫靶点,使用功能活性充分验证的重组蛋白作为抗原免疫兔子,直接从阳性兔B细胞中克隆IgG,重组表达进行流式验证。这一技术不仅大大缩短了研发的时间,也让我们能够直接获得IgG的基因序列信息,而无需担心杂交瘤细胞丢失。



the process of rabbit monoclonal antibody production


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高亲和力、高特异性的兔流式单抗

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图 1. A) Flow cytometry data of serially titrated Rabbit anti-SIRPα monoclonal antibody (clone: DM8). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used. B) Competition assay demonstrating DM8 blockade of CD47 binding to THP-1 cells. IC50=30.88ng/ml.

单个靶点多个候选克隆株,选择度高

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图 2. Affinity ranking of different Rabbit anti-CS1 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

SKU:  DMC101338P      Target:  SEZ6

Application:  Flow Cyt

Price: 100 test ¥3880.00

SKU:  DMC101338B     Target:  SEZ6

Application:  Flow Cyt

Price: 10μg ¥1030.00 ; 50μg ¥3380.00 ; 100 μg ¥6080.00 ; 500 μg ¥19880.00

SKU:  DMC101338     Target:  SEZ6

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101301     Target:  MELTF

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101300     Target:  IBSP

Application:  ELISA

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101299     Target:  ADAM15

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101298     Target:  DLK1

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

Monoclonal Antibodies

Anti-BTC antibody(1F4), Rabbit mAb

SKU:  DME101297     Target:  BTC

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101296     Target:  MST1R

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101295     Target:  NRP1

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101294     Target:  MC4R

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101293     Target:  ECSCR

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101292     Target:  GPR20

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101291     Target:  GLP1R

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101290     Target:  GRPR

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101289     Target:  LRP10

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101288     Target:  APLP2

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

Monoclonal Antibodies

Anti-SPATA46 OTI2A9;Mouse mAb

SKU:  DME911453     Target:  SPATA46

Application:  IHC, WB

Price: 100ul ¥1999

SKU:  DMC101406     Target:  His Tag

Application:  ELISA

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00

SKU:  DME101219     Target:  BTN3A3

Application:  Flow Cyt

Price: 10μg ¥690.00 ; 50μg ¥2280.00 ; 100 μg ¥4080.00 ; 500 μg ¥13280.00