| Clone ID | 12H7 |
|---|---|
| Target | |
| Synonyms | GGGGS |
| Host Species | Rabbit |
| Description | Anti-(G4S)4 antibody(12H7), Rabbit mAb |
| Uniprot ID | N/A |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | N/A |
| Applications | ELISA |
| Recommended Dilutions | ELISA 1/5000-10000 |
| Delivery | In Stock |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect antibody fragments (scFvs) and fusion proteins . The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or 20-mer (G4S)4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains. |
| Usage | Research use only |
| Conjugate | Unconjugated |
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