Human GPR75-Strep full length protein-synthetic nanodisc

Name: Human GPR75-Strep full length protein-synthetic nanodisc - 专注肿瘤免疫
SKU: FLP120031

Price: 10 μg ￥5670.00 ;50 μg ￥27000.00 ; 100 μg ￥49500.00

SKU： FLP120031 分类： Full Length Transmembrane Proteins 标签： synthetic nanodisc

Datasheet

MSDS

Each batch undergoes strict quality control and contact us to know the details.

Product Data Negative control Dima FAQ

Tag	C-Flag&Strep Tag
Target	GPR75
Synonyms	GPRchr2; WI31133
Description	Human GPR75-Strep full length protein-synthetic nanodisc
Delivery	6~8weeks
Uniprot ID	O95800
Expression Host	HEK293
Protein Families	Druggable Genome, GPCR, Transmembrane
Protein Pathways	N/A
Molecular Weight	The human full length GPR75-Strep protein has a MW of 59.4 kDa
Formulation & Reconstitution	Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor.
Usage	Research use only
Conjugate	Unconjugated

图片 Negative control Dima FAQ

Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag GPR75-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-GPR75 monoclonal antibody (DMC100368) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPR75 monoclonal antibody binding with GPR75-Strep-Nanodisc is 15.14ng/ml.

Figure 2. Human GPR75-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE

Figure 3. WB analysis of GPR75-Strep-Nanodisc with anti-GPR75 monoclonal antibody (DMC100368), followed by Goat Anti-Human IgG HRP at 1/5000 dilution

Figure 4. Loaded Human GPR75 monoclonal antibody (DMC100368) on Pro-A Biosensor, can bind human GPR75-Strep full length protein-synthetic nanodisc with an affinity constant of 5.02nM as determined in SPR assay.

Human GPR75-Strep full length protein-synthetic nanodisc

Product Data Negative control Dima FAQ

图片 Negative control Dima FAQ

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