Tag | C-Flag&Strep Tag |
---|---|
Target | |
Synonyms | PTC; T2R38; T2R61; THIOT |
Description | Human TAS2R38-Strep full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | P59533 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Taste transduction |
Molecular Weight | The human full length TAS2R38-Strep protein has a MW of 37.9 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | A seven-transmembrane G protein-coupled receptor that controls the ability to taste glucosinolates, a family of bitter-tasting compounds found in plants of the Brassica sp. Synthetic compounds phenylthiocarbamide (PTC) and 6-n-propylthiouracil (PROP) have been identified as ligands for this receptor and have been used to test the genetic diversity of this gene. Although several allelic forms of this gene have been identified worldwide, there are two predominant common forms (taster and non-taster) found outside of Africa. These alleles differ at three nucleotide positions resulting in amino acid changes in the protein (A49P, A262V, and V296I) with the amino acid combination PAV identifying the taster variant (and AVI identifying the non-taster variant). |
Usage | Research use only |
Conjugate | Unconjugated |
Human TAS2R38-Strep full length protein-synthetic nanodisc
Price: 10 μg ¥5670.00 ;50 μg ¥27000.00 ; 100 μg ¥49500.00
Product Data Dima FAQ
图片 Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag TAS2R38-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TAS2R38-Strep-nanodisc is 8.159ng/ml.
Figure 2. Human TAS2R38-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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