Tag | C-Flag Taq |
---|---|
Target | |
Synonyms | ORXR1; OX1R; OXR1 |
Description | Human HCRTR1 full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | O43613 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Molecular Weight | The human full length HCRTR1 protein has a MW of 47.5 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. |
Usage | Research use only |
Human HCRTR1 full length protein-synthetic nanodisc
Price: 10 μg ¥6300.00 ;50 μg ¥30000.00 ; 100 μg ¥55000.00
Product Data
图片
Figure1. Elisa plates were pre-coated with Flag Tag HCRTR1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with HCRTR1-Nanodisc is 4.139ng/ml.
Figure2. WB analysis of Human HCRTR1-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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