Human CD47 full length protein-synthetic nanodisc

Name: Human CD47 full length protein-synthetic nanodisc - 专注肿瘤免疫
SKU: FLP100039
Price: 8000.00 CNY
Availability: InStock

Price: 10 μg ￥6300.00 ;50 μg ￥30000.00 ; 100 μg ￥55000.00

SKU： FLP100039 分类： Full Length Transmembrane Proteins 标签： synthetic nanodisc

Datasheet

MSDS

Each batch undergoes strict quality control and contact us to know the details.

Product Data Negative control Dima FAQ

Tag	C-Flag Tag
Target	CD47
Synonyms	IAP; MER6; OA3
Description	Human CD47 full length protein-synthetic nanodisc
Delivery	In Stock
Uniprot ID	Q08722
Expression Host	HEK293
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ECM-receptor interaction
Molecular Weight	The human full length CD47 protein has a MW of 35.2 kDa
Formulation & Reconstitution	Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Storage & Shipping	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Usage	Research use only
Conjugate	Unconjugated

图片 Negative control Dima FAQ

FLP100039-CD47-Fig.1-Elisa-1.jpg
Figure 1. Elisa plates were pre-coated with Flag Tag CD47-Nanodisc (0.2μg/per well). Serial diluted anti-CD47 monoclonal antibody (BME100050) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD47 monoclonal antibody binding with CD47-Nanodisc is 2.959ng/ml.
FLP100039-CD47-Fig.2-SDS-PAGE-1.jpg
Figure 2. Human CD47-Nanodisc, Flag Tag on SDS-PAGE

Figure 3. Loaded Human CD47 full length protein-synthetic nanodisc (FLP100039) on Carboxylation Biosensor, can bind Anti-CD47 Magrolimab mAb (BME100050) with an affinity constant of 0.17nM as determined in SPR assay.

Human CD47 full length protein-synthetic nanodisc

Product Data Negative control Dima FAQ

图片 Negative control Dima FAQ

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