Human CD40 Ligand Protein, hFc Tag
|Package||10 μg ￥650.00 ; 50 μg ￥2600.00 ; 100 μg ￥3900.00|
|Description||Recombinant human CD40 Ligand Protein with N-Human Fc tag|
|Tag||N-Human Fc tag|
|Molecular Weight||The protein has a predicted molecular mass of 42.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-CD40 Ligand is approximately 40-53 kDa due to glycosylation.|
|Purity||The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.|
|Formulation & Reconstitution||Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.|
|Storage||Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).lyophilized proteins are shipped at ambient temperature.|
|Usage||Research use only|
Figure 1. Human CD40 Ligand Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40 Ligand,hFc tagged protein (PME100192) can bind Human CD40, mFc-His tagged protein (PME100015) in a linear range of 0.51-320 ng/ml.
Figure 3. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CD40L, hFc tagged protein (PME100192) can bind Anti-CD40L Neutralizing antibody (BME100042) in a linear range of 0.64-400 ng/ml.
|Background||The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.|